Product Description
The Real-Time Quantitative PCR Systems, models YR06248 and YR06249, are advanced laboratory instruments designed for precise genetic analysis. These systems boast cutting-edge technology, offering a remarkable sample capacity: they can accommodate up to 96 individual tubes or a 0.1mL PCR plate. With a flexible sample volume range from 10 to 50 μL, these systems provide researchers with the versatility needed for a wide range of experiments.
Heating and cooling are efficiently managed using the Peltier method, achieving a maximum ramping rate of 6.0°C/s. This ensures rapid cycling and shortened experimental times. The systems operate within a temperature range of 4-100°C, maintaining exceptional accuracy and uniformity at ±0.2°C and ±0.2°C@60°C/±0.3°C@95°C, respectively. This precision is critical for reproducible results in PCR assays.
| Model | YR06248 | YR06249 |
| Sample capacity | 8*0.1mL PCR strip*12 96*0.1mL individual tube 0.1mL PCR Plate |
|
| Sample volume | 10-50 μL | |
| Heating and Cooling method | Peltier | |
| Maximum ramping rate | 6.0° C/s | |
| Temperature range | 4-100 °C | |
| Temperature accuracy | ± 0.2°C | |
| Temperature uniformity | ±0.2°C@60°C ±0.3°C@95°C | |
| Optical Detecting System | ||
| Excitation light | 4pcs high-efficiency single color LED | 5pcs high-efficiency single color LED |
| Detector | SiPMT | |
| Detection method | Time-resolved real-time scanning | |
| Excitation/Emission wavelengths | 455-650nm | 510-750nm |
| Detection Channels | 4 (optional 5 channels) | |
| Supported dyes | FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ, (Optional- Cy5.5 for 5-channel model) | |
| Multiplexing | Up to 4 targets (optional 5 targets for 5-channel model) | |
| Sensitivity | 1 copy gene | |
| Resolution | 1.33-fold copies difference in single-plex reaction | |
| Dynamic range | 10 orders of magnitude | |
Market Pricing
The market for Real-Time PCR Systems is competitive, with prices typically ranging from approximately $22,500 to $23,700 USD. Our models offer exceptional value, combining advanced features with reliability, making them a top choice for laboratories seeking high-quality, cost-effective solutions.
Frequently Asked Questions
What is included in the purchase of the PCR system?
The purchase includes the PCR unit, a power supply, and a comprehensive user manual to ensure optimal operation from day one.
Is the system compatible with different dye types?
Yes, the system supports a variety of dyes including FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, and Cy5/LIZ. An optional upgrade allows for Cy5.5 if using a 5-channel model.
How user-friendly is the operating software?
The software is designed to be intuitive, allowing users to program runs and analyze data with minimal training. Its advanced capabilities do not compromise ease of use.
Advantages and Disadvantages
The Real-Time Quantitative PCR Systems offer several advantages. These include high precision in temperature management, robust multiplex capabilities, and compatibility with a wide range of dyes, making them suitable for various applications. The sensitive detection is capable of identifying single gene copies, thus ensuring accurate genetic analysis.
However, a potential disadvantage might be the initial cost. While the system offers an excellent return on investment due to its features and reliability, the upfront cost may be higher than more basic models on the market. Despite this, the advanced capabilities justify the investment for laboratories with demanding workloads.
Field Application
In practical settings, the Real-Time PCR Systems are utilized for genetic analysis in various areas such as biomedical research, diagnostics, and agriculture. Their capacity to handle multiple sample types and their flexibility in programming cycles make them ideal for high-throughput environments.
Researchers benefit from the system’s precision and the speed of its temperature ramping, which reduces the time needed for analysis and increases the throughput capacity. The robust detection system also ensures high sensitivity, providing reliable data for complex analyses.
Recommendations
To maximize the performance of the PCR system, it is essential to ensure proper maintenance, which includes routine calibration and software updates. Furthermore, users should utilize the recommended sample volumes and adhere to the protocol guidelines to achieve the best results.
It is also advisable to secure the system in a stable environment, where temperature fluctuations are minimal, to maintain the accuracy of the assays. Engaging in regular training updates for staff can further enhance the operational efficiency and longevity of the equipment.
Are you ready to enhance your laboratory’s capabilities with this state-of-the-art PCR system? Request a quote now or generate a quote automatically through the Kalstein Plus platform for unrivaled value and performance.
If you’re looking for a fusion of innovation and quality, you have come to the right place. At Kalstein, we offer you the luxury of exploring our exclusive catalog of laboratory equipment. We manufacture each piece of equipment with a level of excellence. Our intuitive and agile online purchasing channels are designed for your convenience, ensuring the most friendly prices. Don’t hesitate any longer, we bring science to life, it’s time to be part of our community. https://kalstein.net/en/product/real-time-quantitative-pcr-system-yr06248-yr06249/.
