Kalstein

Multimode Microplate Reader YR07004

SKU:YR07004 · By Kalstein

Category:Microplate reader

Multimode Microplate Reader YR07004

The YR07004 is a high-end multimode microplate reading system (ABS, FL, LUM, TRF) specifically designed for medical, biological, and pharmaceutical research ins

The multimode microplate reader YR07004 offers exceptional processing capabilities through its advanced monochromator system and specialized optics with high-sensitivity photomultiplier tubes. The equipment allows for rapid spectral scanning, quantification of nucleic acids without dilution using u-Nano plates, and assays with dual automatic injectors. Its 10 GB data storage capacity and advanced connectivity via Ethernet, USB, and FTP protocols optimize information transfer. The device's structure is designed to ensure a long lifespan, low operational noise, and seamless integration into hospital management networks. It represents a cutting-edge comprehensive solution for laboratories seeking maximum efficiency and analytical reliability.

Advanced Design and Technology

  • 4 Mode Versatility: Offers complete detection solutions including absorbance (UV/Vis), fluorescence, luminescence, and time-resolved fluorescence (TRF).

  • High Precision Optics: Utilizes an advanced monochromator system for absorbance (200 to 1000 nm) and filter-based optics with dichroic mirrors for fluorescence, ensuring high light transmission and sensitivity.

  • Control and Luminescence Performance: Incorporates a photomultiplier tube (PMT) optimized for weak signals, preventing saturation and minimizing crosstalk between wells (crosstalk ≤0.005%).

  • Ultramicro u-Nano Plate: Allows for rapid, undiluted quantification of 16 or 96 nucleic acid and protein samples simultaneously using a minimum volume of 2 to 4 μL.

  • Dual Automatic Injector (Optional/Integrated): Designed for instant luminescence assays and $Ca^{2+}$ flow, with software protection to prevent overflow.

Frequently Asked Questions (FAQ)

  • What types of plates does the instrument support? It is compatible with a wide range of supports, including 6, 12, 24, 48, 96, and 384 well plates, PCR plates, and ultramicro u-Nano plates.

  • How is spectral scanning performed in absorbance? It employs a long-lasting xenon lamp (up to $10^9$ flashes) capable of scanning the entire spectrum of a sample between 200 and 1000 nm in 1 nm increments in less than 15 seconds.

  • Can data be managed remotely? Yes, the test data report can be directly uploaded to a PC server via an Ethernet connection and FTP protocol.

Advantages and Disadvantages

Key Advantages:

  • Extreme Sensitivity: Highly optimized detection limits, such as 1 pM in fluorescence and up to 15 amol/well in luminescence.

  • Dynamic Stirring: Features three independent stirring modes (linear, circular, and doubly circular) with adjustable speed to optimize kinetic reactions.

  • Intelligent Interface: 10-inch capacitive touchscreen operated via the ReaderIt-II analysis software and fully compatible with 64-bit Windows 10/11.

Disadvantages / Considerations:

  • Infrastructure Requirement: As a high-end equipment with multiple optical and mechanical modules (33 kg), it requires a stable and dedicated work surface.

  • Calibration Curve: The use of multiple analytical modes and advanced accessories (such as the automatic injector) requires personnel with specific technical training for full utilization of the system.

Product Use in the Field and Recommendations

  • Ideal Applications: Research laboratories in life sciences, pharmaceutical quality control, trace analysis in biomedicine, immunoassays, and gene expression studies.

  • Recommended Maintenance: Periodically check the status of the modular filters using QR codes to ensure parameter accuracy and clean the automatic injector conduits after assays with reactive samples.

  • Operation Recommendation: Use the optical performance validation plate for ABS during installation and operational qualification stages to ensure traceability of absorbance measurements.

ModelYR07004
ABS
Plate6 well plate, 12 well plate, 24 well plate, 48 well plate, 96 well plate, 384 well plate, 96/384 PCR plate, 16/96 ultramicro u-Nano plate
Reading time (spectrum)200~1000 nm <15 s (1 nm step)
Light sourceXenon lamp
Wavelength accuracy2 nm
Wavelength repeatability (SD)0.2 nm
Half width (FWHM)<2.5 nm
DetectorPD×2
Wavelength range200~1000 nm, 1 nm step
Measurement range0-4 DE
Resolution0.0001 DE
Accuracy at 450 nmPrecision mode: ±(1.0%+0.003OD) @(0.0-2.0OD]; ±2%>@(2.0-3.0OD]
Repeatability at 450 nmCV<1.0% or SD<0.003 fast (0.0-3.0 OD]; CV<0.5% or SD<0.003 precise (0.0-3.0 OD]
Scattered light0.1 % at 220 nm
Linear at 450 nmR²≥0.999 @ [0.0 - 3.0 OD]
Reading time96 well plate: fast <15 s (A1 to H1)
Fluorescence
Reading modeHighlighted readings
Excitation light sourceXenon lamp
DetectorPMT
Wavelength rangeEX: 200~1000 nm; EM: 270~850 nm
EX / EM filterEX485 / EM530, EX523 / EM564, EX624 / EM692 (other wavelengths can be substituted)
Linear dynamic range≥6 logs
Detection limit1 pM (optimization condition)
LUMINESCENCE
DetectorPMT
Detection limit≤15 amol/well on 96 well white plate with ATP flash
Linear dynamic range≥6 logs
Crosstalk≤0.005%
Wavelength range200-850 nm
FilterLUMINESCENCE
TRF
EX / EM filterEX365 / EM612
Detection limit0.02 pM (optimization condition)
Software
Analysis softwareReaderIt-II Software
Basic parameter
Reading typeEnd point, kinetics, spectral scanning, and well scanning
Plate6 well plate, 12 well plate, 24 well plate, 48 well plate, 96 well plate, 384 well plate, 96/384 PCR plate, 16/96 ultramicro u-Nano plate
Accessories16/96 ultramicro u-Nano plate, auto-injector
Stirring modeLinear, circular, double circular (force and speed adjustable)
Incubation temperatureTA. +4 °C~45 °C
Temperature accuracy±0.5 °C at 37 °C
Software interfaceChinese / English
Screen size10 inches
Operation methodCapacitive touchscreen, mouse
Data capacity10 GB
CompatibilityCompatible with PC software, Windows 10 and later versions, 64 bits.
Network transmissionThe test data report can be uploaded to the PC server via FTP.
Instrument ports2 USB A ports, 1 USB B port, 1 Ethernet port, RS232 bus interface (connected to the injector)
Power supplyAC 100-240 V, 50-60 Hz
Packing dimensions80×78×80 cm
Weight33 kg
Accessory parameter
u-Nano
Sample numberu-Nano 16: 1~16, u-Nano 96: 1~96
Sample detection volume2-4 μL
Automatic injector
Support plate6-384 well plate
Quantity1/2
Dispensing volume5-1000 μL, 1 μL increment
Liquid injection speed125-500 μL/s
Accuracy±1 μL at 5-50 μL; ±2% at 51-1000 μL
Residual liquid collection25 ml

Technical specifications

ManufacturerKalstein

Video Multimode Microplate Reader YR07004

Frequently asked questions

How to know the prices of Multimode Microplate Reader YR07004?

To know the price of Multimode Microplate Reader YR07004, please send us an email with your request through the contact form AQUI.

What are the delivery times for Multimode Microplate Reader YR07004?

Delivery time depends on stock availability and freight type (air or sea). In stock: air 15-30 days, sea 45-60 days. Out of stock: air 30-60 days, sea 60-90 days.

How to make a purchase of Multimode Microplate Reader YR07004?

You can buy by email ([email protected]), phone (+33 (0) 1 70 39 26 50) or through the official Kalstein website in your country.

How does the warranty work for Multimode Microplate Reader YR07004?

All Kalstein equipment comes with a 1-year warranty against manufacturing defects. The warranty does not cover damage from improper installation or misuse. See our «terms and conditions» AQUI.

Can I request a quote online for Multimode Microplate Reader YR07004?

Yes, you can request a quote for the Kalstein equipment you are interested in directly from our official website. Click AQUI.