Product Description
The Real-Time Quantitative PCR System YR06248 and YR06249 are cutting-edge laboratory instruments designed to meet the demands of modern genetic analysis. These models are carefully crafted to offer precision and reliability in DNA amplification and detection. Equipped with a comprehensive sample capacity, these systems can accommodate 96 individual tubes of 0.1mL, 0.1mL PCR plates, and 8 strips of 0.1mL PCR, ensuring a broad range of experimental possibilities. The migration of samples ranging from 10 to 50 μL allows for flexibility in numerous applications.
Both models utilize the Peltier heating and cooling method, achieving a maximum ramping rate of 6.0°C per second. The systems maintain a temperature range from 4°C to 100°C, with an accuracy of ±0.2°C and a uniformity of ±0.2°C at 60°C and ±0.3°C at 95°C, emphasizing their effective heat control. The optical detecting system in these devices is powered by high-efficiency single-color LEDs, with the YR06248 model using four LEDs and the YR06249 using five. The detection method is time-resolved real-time scanning, guaranteeing precise results across a range of excitation and emission wavelengths.
| Model | YR06248 | YR06249 |
| Sample capacity | 8*0.1mL PCR strip*12 96*0.1mL individual tube 0.1mL PCR Plate |
|
| Sample volume | 10-50 μL | |
| Heating and Cooling method | Peltier | |
| Maximum ramping rate | 6.0° C/s | |
| Temperature range | 4-100 °C | |
| Temperature accuracy | ± 0.2°C | |
| Temperature uniformity | ±0.2°C@60°C ±0.3°C@95°C | |
| Optical Detecting System | ||
| Excitation light | 4pcs high-efficiency single color LED | 5pcs high-efficiency single color LED |
| Detector | SiPMT | |
| Detection method | Time-resolved real-time scanning | |
| Excitation/Emission wavelengths | 455-650nm | 510-750nm |
| Detection Channels | 4 (optional 5 channels） | |
| Supported dyes | FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ，（Optional- Cy5.5 for 5-channel model） | |
| Multiplexing | Up to 4 targets (optional 5 targets for 5-channel model) | |
| Sensitivity | 1 copy gene | |
| Resolution | 1.33-fold copies difference in single-plex reaction | |
| Dynamic range | 10 orders of magnitude | |
Market Price
The Real-Time Quantitative PCR Systems YR06248 and YR06249 are competitively priced within the advanced laboratory equipment market. Typically, you can expect the price of such sophisticated equipment to range between $22,700 to $23,300, reflecting the balance between cutting-edge technology and industry demands. These devices represent a sound investment for laboratories looking to enhance their genetic analysis capabilities.
Frequently Asked Questions
What are the key differences between models YR06248 and YR06249?
The primary difference lies in the number of excitation LEDs; YR06248 features four, while YR06249 boasts five for enhanced detection capabilities.
Can these systems handle multiplexing?
Yes, both models can multiplex up to 4 targets, with an optional expansion to 5 targets for the 5-channel model.
What is the sensitivity level of these PCR systems?
The systems are highly sensitive, capable of detecting as low as a single copy gene.
Advantages and Disadvantages
Advantages:
- High precision with temperature accuracy and uniformity.
- Robust optical detection system for a broad range of applications.
- Versatile sample capacity for diverse experimental designs.
- Intuitive operation and reliable results due to time-resolved real-time scanning.
Disadvantages:
- Initial cost can be a factor for small labs with limited budgets.
- Requires skilled personnel for optimal operation and maintenance.
Product Usage in the Field
The Real-Time Quantitative PCR System YR06248 and YR06249 are indispensable tools in diverse fields ranging from clinical diagnostics to agricultural biotechnology. In clinical settings, they facilitate rapid and accurate pathogen detection and quantification in various samples. In research laboratories, these systems enable detailed gene expression studies, crucial for advancing our understanding of genetic markers. Their high sensitivity and multiplexing capability make them ideal for environments necessitating quick turnaround and precise data.
Recommendations
To maximize the effectiveness of the YR06248 and YR06249 models, regular calibration and maintenance are essential. Ensuring the use of high-quality reagents and strict adherence to operating protocols will significantly contribute to the reliability and accuracy of results. Providing adequate training for lab personnel on the functionalities and troubleshooting techniques will also enhance productivity and mitigate the risk of user error. For those not yet using advanced laboratory equipment, consider investing in these PCR systems for cutting-edge research and diagnostics.
If you are in search of a fusion of innovation and quality, you have come to the right place. At Kalstein, we offer you the luxury of exploring our exclusive catalog of laboratory equipment. We manufacture each piece with a level of excellence. Our online purchasing channels, intuitive and agile, are designed for your convenience, ensuring the friendliest prices. Don’t hesitate any longer; we bring science to life, and it’s time for you to be part of our community. https://kalstein.net/en/product/real-time-quantitative-pcr-system-yr06248-yr06249/.
