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We give our customers a better option when they use our thermal cells, backed by excellent service and support. Thermal Cyclers are used for qualitative amplifications or to quantify the amount of amplified DNA. The results of these amplification techniques have a substantial impact on our society; so it should be ensured that thermoci-clues are accurate; Exact and uniform, in order to get reliable results.
The PCR reaction requires the presence of Mold, primers, nucleotides and DNA polymerase. DNA polymerase is the key enzyme that using DNA mold makes a copy of this by incorporating nucleotides sequentially into the product of the PCR. Adenine, thymine, cytosine and guanine nucleotides are the blocks of the resulting new copy.
Primers or oligonucleotides are those that confer specificity to the reaction since they are short DNA fragments with a defined sequence complementary to the target DNA that wants to be amplified. The DNA polymerase uses the primers as a starting point of the polymerization of the new DNA fragment.
The Thermal Cyclers is a computer that allows the chain reaction of the polymerase (PCR) efficiently and quickly; by means of the automatic and cyclic embodiment of the temperature changes required for the amplification of a deoxyribonucleic acid chain (DNA); from a thermostable enzyme.
Thermal cells are used for qualitative amplifications or to quantify the amount of amplified DNA. The results of these DNA amplification techniques have a great impact on our society; Especially in this pandemic period where they have become one of the main diagnostic tests, which is why it should be ensured that thermocyckers are accurate; Exact and uniform, in order to obtain reliable results.
The most common model consists of a block of electrical resistance that distributes a homogeneous temperature through a plate during times that can be programmable, usually with temperature ranges of 4 ° C to 96 ° C where denaturation, hybridization and extension of A DNA molecule.
The components of the PCR are mixed in a test tube and then placed on a machine that allows repeated DNA amplification cycles to occur. The machine Thermal Cyclers with a thermal block, in which the tubes are inserted. Thermal Cyclers elevates and lowers the block temperature in three preprogrammed basic stages. The reaction is heated for the first time above the denaturation point of the two complementary DNA chains of the target DNA, which allows the threads to be separated.
The temperature is then lowered to allow specific primers to join the target DNA segments, a process known as hybridization. The temperature rises again to a temperature at which the DNA polymerase is capable of extending the primers by adding nucleotides to the DNA strand that is being built.
If we take it from the perspective of a molecular biologist, this is the best way to calibrate any thermocycler as long as it is the process that mimics the PCR process. The correct process is to put tuvos in block wells, fill the reaction mixture and place the sensors. Then proceed to close tubes and thermal cyclers with the thermal cover in order to develop the PCR protocol...
From a perspective, the best way to calibrate a utensil such as the thermal cycler is by representative and standard measurement of the PCR process. The method allows the calibration of all standard PCR. The dynamics and its static part must be taken into account during the PCR process. This is important since there are problems that cannot be known with the naked eye, or rather with the static control of a PCR.
During this process traceability is guaranteed by making use of reference standards and expression of values that are responsible for calibrating in SI units. However, also because of that uncertainty calculated from the calibration result. In addition to the fact that the same calibrations are only made by engineers specialized in calibration who are literally competent and qualified to perform such action.
The thermal cycler or thermal sequencer is a device that allows polymerase chain reaction (PCR) efficiently and quickly, by automatic and cyclic realization of the temperature changes that are required for the amplification of a deoxyribonucleic acid chain ( DNA), from a thermostable enzyme.
That is why the calibration must be carried out by qualified personnel, that is, no ordinary person can try it. Remember that years of study are carried out for this. The views should also be carried out under defined environmental conditions that allow the proces...