Electrophoresis is an analytical method in which a controlled electric current is used in order to separate biomolecules according to their size to electric charge ratio, using a gelatinous matrix as a base. This technique has a variety of practical uses, such as forensic medicine for human identification, the human genome project, protein and genetic mutation research, and clinical diagnostic testing.
Electrophoresis is carried out with equipment composed of a negative charge at one end and a positive charge at the other, called an electrophoresis system. When inserting charged molecules, in this environment, the negative ones will go to one extreme and the positive ones to the other corresponding. For example, when analyzing proteins on a gel, in these kits, the entire protein is taken to analyze its size. In this way, the shorter ones will migrate to the poles more quickly and will be reflected in the lower part of the gel. Instead, the longest will be at the top.
Horizontal gel electrophoresis uses the basic theory for the separation of DNA, RNA or protein molecules according to their respective molecular size and charge. In this technique, the gel is present in a horizontal orientation and is immersed in a buffer that is continuous. The agarose gel is used to separate the gel box into two compartments.
One end of the gel box contains an anode, while the other end contains a cathode. When a current is applied, the buffer used in this technique allows the creation of a charge gradient. When the load is applied, the gel tends to heat up.
The buffer also functions as a coolant, keeping the temperature at optimal levels. Recirculation of the running buffer prevents the formation of a pH gradient. A discontinuous buffer system cannot be used in horizontal gel electrophoresis as the two compartments of the gel system connect with the running buffer.
The vertical gel electrophoresis technique works according to the primary theory of gel electrophoresis, but is considered to be more complex than the horizontal gel electrophoresis method. This technique uses a discontinuous buffer. A cathode is located in the upper chamber, and the anode is located in the lower chamber. The electrodes present in each compartment provide the required electric field. A thin layer of gel is poured between the two mounted glass plates.
Therefore, the upper part of the gel is immersed in the upper chamber, and the lower part of the gel is immersed in the lower chamber. Once the current is applied, a small portion of the buffer moves to the lower chamber from the upper chamber through the gel.
In vertical gel electrophoresis, the buffer only flows through the gel. This allows precise control of the voltage gradient during the separation stage. Acrylamide gel can be used as the compartments are not exposed to atmospheric oxygen. Due to the smaller pore size of the acrylamide gel, precise separation can be achieved with higher resolution.
You can adjusting the position of the adjusting rod according to the medium length, to meet the different...
Vertical gel electrophoresis is a more complex set-up compared to horizontal gel system. Researchers normally...
Vertical gel electrophoresis is a more complex set-up compared to horizontal gel system. Researchers normally ...
YR horizontal gel electrophoresis units have been designed by scientists with the laboratory environment in mind...
Vertical gel electrophoresis is a more complex set-up compared to horizontal gel system. Researchers normally use this system to separate proteins instead of nucleic acids. However, before you separate proteins, you need to disrupt the quaternary structure of proteins into the linear strand
Characteristic features
| Model | YR03428 |
| Glass plate size（W×L） | 216×110 mm |
| Gel Size（W×L） | 186×95 mm |
| Gel thickness | 1.0（mm） |
| Gel amount | 1～2（pieces） |
| Sample volume | （1.0mm）25、40、52wells |
| Buffer Volume | ～1800（ml） |
| Dimension（L×W×H） | 280×140×135（mm） |
| Net weight | 3.2（kg） |
Electrophoresis is an analytical method in which a controlled electric current is used in order to separate...
Electrophoresis in agarose or polyacrylamide gels is one of the methodologies most used in the laboratory...
Gel electrophoresis is a group of techniques used in order to separate molecules, based on their properties such...
Electrophoresis consists of a technique that allows the separation of biomolecules according to their mobility...
A power source for electrophoresis is an electrical equipment that has the function of managing the current in a constant ...
Strictly speaking, a paternity test is not a genetic test because it provides little information about inherited genes ...
Undoubtedly, the possibility of using molecular genetics in the field of veterinary sciences and agriculture, is that the production ....
The power source for electrophoresis chambers is the source in charge of providing electricity to the electrophoresis system that is being used, in other words, it provides the necessary energy for this system to function properly; reason for which it is...
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