The cryostat rotating and cutting mechanism is located inside a refrigerated chamber, at a temperature between -20 and -30 °C. In this chamber is the sample, the blade and is where the sections are collected, process by which the sections are glued to a slide. Freezing is usually done on a platform inside the chamber itself cooled by Peltier's system. The tissue can also be frozen externally as quickly as desired, e.g. with liquid nitrogen, but it is advisable to place the sample in the cryostat chamber until it can match its temperature to that of the cryostat to obtain homogeneous sections.
Before freezing, the sample is embedded in a medium that is liquid at room temperature and solid at the cutting time. Therefore, we have our sample in a solid block, embedded but not included. This allows the sample to be manipulated and attached to a sample holder, which will be fixed to an axis that advances on the blade.