A spectrophotometer is a device used in laboratories to determine what the beam of radiation or electromagnetic light is and thus identify, qualify and quantify its intensity. It also allows to determine its efficiency, sensitivity, resolution and spectral range. This will depend on the variability of the design and the choice of optical components it contains.

Its operation is based on the light of the special lamp it has, which is guided by a connector that selects and separates the light from the wavelength, and then passes through a sample. The intensity of the light coming out of that sample is captured and compared with the intensity of the light that affects that sample, with this information the transmittance can be calculated, which will depend on the concentration of the substance.

Types of Spectrophotometers a laboratory may need

Single-beam Spectrophotometers

The light is transported from the sample to the detector, so a reference is needed to perform the analysis. Spectrophotometers are widely used in biomedical sciences and life sciences research, including university and industrial research. Typical uses of the spectrophotometer are measurements of the density of nucleic acids, proteins and bacteria.

However, spectrophotometers are also widely used in various industries, such as biotechnology, diagnostic and clinical testing, drug research, pharmaceutical research, chemical engineering, materials science and agricultural research.

Double beam Spectrophotometer

Light travels through two paths into the compartments, each with its own detector. One goes to the sample and the other to the reference. These devices have the ability to project a monochromatic light beam through a sample and measure the amount of brightness absorbed by that sample. As a result, the spectrophotometer can provide us with information on the nature of the substance in the sample, as well as indirectly indicate how much of the substance we are interested in will be present in the sample.

Some of the essential components of the spectrophotometer are, for example, the light source which illuminates a given sample and which must respond to a number of conditions such as, for example, directionability, stability, continuous spectral energy distribution. Typically used sources are the tungsten lamp or the xenon arc lamp.


In Kalstein you can find the ideal spectrophotometer for your laboratory

There are a myriad of models, so it's normal that you don't know which spectrophotometers to buy to meet your needs. At Kalstein, we evaluate them to find what you're looking for.

VIS UV Spectrophotometer with scanning software

Innovative optical design and the latest generation monochromator with high-grade flame holographic grille ensure...


UV/VIS Visible Ultraviolet Spectrophotometer - YR01854

Large LCD screen (128×64 dots). You can display a total of 200 data groups, 5 groups per screen...


UV/Vis double beam spectrophotometer - YR01858

The powerful data analysis system with an internal computer host could perform multiple...


Portable Desktop Spectrophotometer YR05485

YR05485 portable desktop spectrophotometer color measurement instrument features with repeatability...


Our best-selling Spectrophotometer

  • Dual optical path, dual beam optical system, dual detector, high performance imported grid, lower parasitic light, greater stability, reliability, more accurate analysis
  • Equipped with 10" touch screen, built-in 32G memory, Bluetooth network support
  • Dynamic functions, DNA/protein testing, multiple wavelength testing and data printing
  • Adopt the suspension design of the optical system, the entire optical path is set independently on the deformation-free base of aluminum 16 mm thick
  • Vibration from the outside has no impact on the optical system, greatly improving the stability and reliability of the instrument

Analysis of the best Spectrophotometers for your laboratory

Spectrophotometers: What are the main brands?

A spectrophotometer is a laboratory instrument used to measure the absorbance of a sample, depending on the...


What is a UV-VIS spectrophotometer?

UV-VIS spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light...


When to calibrate my spectrophotometer?

The calibration process, according to instructions for use, should be performed at most every 14 days. Our recommendation...


What are the uses of a spectrophotometer?

The spectrophotometer quantifies the concentration of a specific substance that is present in a sample...


Laboratory spectrophotometers: an introduction

A spectrophotometer, commonly known as a UV/VIS spectrophotometer, is a device used to measure radiation absorption in the visible...

Spectrophotometers: What are the main brands?

A spectrophotometer is a laboratory instrument used to measure the absorbance of a sample, as a function of the wavelength...

How to keep your spectrophotometer in good condition?

The spectrophotometer is one of the main diagnostic and research instruments developed by humans....

The revolution of measuring light spectra: Spectrophotometry for laboratory

Spectrophotometry is a scientific technique that focuses on the measurement of light. This technique allows scientists to obtain detailed information about the contents of certain substances, taking into account how the difference-frequency radiation is spread through the materials.

Spectrophotometer videos in operation

High accruacy spectrophotometer is used for accurate analysis and transmission of laboratory color.Apply in paints, inks, textiles, garments, printing and dyeing, printing etc industries for color transfer and quality control, also for Fluorescence sample color measurement.

It is used to measure the brightness factor and color coordinates of traffic signs, markings and reflective films. It contains GB 2893 and GB/T 18833 standard colors. It can customize the rectangular tolerance of polygons manually.

Frequently asked questions about Spectrophotometers from our customers

To know the price of Spectrophotometers we invite you to send us an email with your request through the contact form.

The delivery time of your  Kalstein product  will depend on the following: 

  1. If the equipment of your interest is in stock or if on the contrary it should be manufactured. 
  2. The type of freight you have chosen may be; air or sea.
  • Equipment in stock:  

- Delivery Time ( Aereo ):  15 -30  days .  

- Delivery Time ( Maritime ): 45-60  days . 

  • Equipment not in stock:  

- Delivery Time ( Aereo ): 30 -60  days .  

- Delivery Time ( Maritime ):  60-90  days . 

Can  realize  your  purchase through : 

  1. By email: sales@kalstein.eu
  2. By phone: +33 (0) 1 78 95 87 02
  3. Trade  electronic : Through  from Kalstein's official website  in your  country . 

All Kalstein equipment has a 1-year warranty against manufacturing defects. The warranty does not cover damage caused by a poor installation or operation by the user, transportation defects or uses other than those specified by the manufacturer. Warranty excludes electrical or consumable parts. For more information visit our "terms and conditions "  by clicking HERE

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