A spectrophotometer is a device used in laboratories to determine what the beam of radiation or electromagnetic light is and thus identify, qualify and quantify its intensity. It also allows to determine its efficiency, sensitivity, resolution and spectral range. This will depend on the variability of the design and the choice of optical components it contains.
Its operation is based on the light of the special lamp it has, which is guided by a connector that selects and separates the light from the wavelength, and then passes through a sample. The intensity of the light coming out of that sample is captured and compared with the intensity of the light that affects that sample, with this information the transmittance can be calculated, which will depend on the concentration of the substance.
The light is transported from the sample to the detector, so a reference is needed to perform the analysis. Spectrophotometers are widely used in biomedical sciences and life sciences research, including university and industrial research. Typical uses of the spectrophotometer are measurements of the density of nucleic acids, proteins and bacteria.
However, spectrophotometers are also widely used in various industries, such as biotechnology, diagnostic and clinical testing, drug research, pharmaceutical research, chemical engineering, materials science and agricultural research.
Light travels through two paths into the compartments, each with its own detector. One goes to the sample and the other to the reference. These devices have the ability to project a monochromatic light beam through a sample and measure the amount of brightness absorbed by that sample. As a result, the spectrophotometer can provide us with information on the nature of the substance in the sample, as well as indirectly indicate how much of the substance we are interested in will be present in the sample.
Some of the essential components of the spectrophotometer are, for example, the light source which illuminates a given sample and which must respond to a number of conditions such as, for example, directionability, stability, continuous spectral energy distribution. Typically used sources are the tungsten lamp or the xenon arc lamp.
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Model | YR01862 | YR01862-1 | |
Wave range | 190-1100 nm | ||
Spectral Bandwidth. | 1.8nm | 0.5, 1.0 2.0, 4.0 nm | |
Accuracy of the wavelength | ± 0.1nm (D2 656.1nm), ± 0.3nm in full range | ||
Light gone | 0.03% T @ 220 nm, 360 nm | ||
Wavelength-repeatability | ≤0.1nm | ||
Photometric accuracy | 士 0.2% T (0-100% T) ± 0.002Abs (0-0.5Abs) ± 0.004Abs (0.5-1.0Abs) |
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Photometric repeatability | ≤0.1% T (0-100% T) 0.001Abs (0-0.5Abs) 0.002Abs (0.5-1.0Abs) |
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Stability | 士 0.0004A/[email protected] | ||
Photometric Range | 0-200% T, -0.3-3.0A, 0-999C | ||
Planitude of the baseline | 士 0.001A | ||
Noise | 士 0.0004A | ||
Monitor | 800*480 Touch Screen | ||
Data-out port | USB | ||
Print Port | USB port | ||
Source of light | Tungsten lamp and deuterium lamp | ||
power requirements | AC 220V / 50Hz or AC 110v / 60Hz | ||
Detector | Silicon photodiode | ||
Dimension | 630 * 430 * 210 nm | ||
Weight | 28KG |
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In this section you can find our spectrophotometers in operation, packed, receiving service, etc.
This short animation shows the inner workings of a spectrophotometer. Practice using a virtual spectrophotometer.
So is as works a spectrophotometer. One lamp provides the source of light. The beam of light hit the grid of diffraction, that works as a prism and separate the light in your lengths of wave components.
The grid be tour of shape that alone one length of wave specific of the light arrives a the slit of exit. Then the light interacts with the sample. A split of east point, the detector measure the transmittance and absorbance of the sample.
Spectrophotometers, in general, are very specialized equipment. Its conservation depends largely on the way of installation and use. The surrounding environment and the quality of electricity services are vital factors in enabling equipment to provide services in accordance with the specifications for which it was manufactured.
Maintenance procedures that may require varying complexity, from thorough cleaning of their components to specialized procedures, shall be performed only by trained technicians or engineers with the technical information developed by the manufacturers.
To know the price of spectrophotometers we invite you to send us an email with your request through the contact form.
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- Delivery Time ( Maritime ): 45-60 days .
- Delivery Time ( Aereo ): 30 -60 days .
- Delivery Time ( Maritime ): 60-90 days .
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