Spectrophotometers
A spectrophotometer is a device used in laboratories to determine what the beam of radiation or electromagnetic light is and thus identify, qualify and quantify its intensity. It also allows to determine its efficiency, sensitivity, resolution and spectral range. This will depend on the variability of the design and the choice of optical components it contains.
Its operation is based on the light of the special lamp it has, which is guided by a connector that selects and separates the light from the wavelength, and then passes through a sample. The intensity of the light coming out of that sample is captured and compared with the intensity of the light that affects that sample, with this information the transmittance can be calculated, which will depend on the concentration of the substance.
Types of spectrophotometers a laboratory may need
Single-beam spectrophotometers
The light is transported from the sample to the detector, so a reference is needed to perform the analysis. Spectrophotometers are widely used in biomedical sciences and life sciences research, including university and industrial research. Typical uses of the spectrophotometer are measurements of the density of nucleic acids, proteins and bacteria.
However, spectrophotometers are also widely used in various industries, such as biotechnology, diagnostic and clinical testing, drug research, pharmaceutical research, chemical engineering, materials science and agricultural research.
Double beam spectrophotometer
Light travels through two paths into the compartments, each with its own detector. One goes to the sample and the other to the reference. These devices have the ability to project a monochromatic light beam through a sample and measure the amount of brightness absorbed by that sample. As a result, the spectrophotometer can provide us with information on the nature of the substance in the sample, as well as indirectly indicate how much of the substance we are interested in will be present in the sample.
Some of the essential components of the spectrophotometer are, for example, the light source which illuminates a given sample and which must respond to a number of conditions such as, for example, directionability, stability, continuous spectral energy distribution. Typically used sources are the tungsten lamp or the xenon arc lamp.
In Kalstein you can find the ideal spectrophotometer for your laboratory
VIS UV Spectrophotometer with scanning software
Innovative optical design and the latest generation monochromator with high-grade flame holographic grille ensure...
UV/VIS Visible Ultraviolet Spectrophotometer - YR01854
Large LCD screen (128×64 dots). You can display a total of 200 data groups, 5 groups per screen...
UV/Vis double beam spectrophotometer - YR01858
The powerful data analysis system with an internal computer host could perform multiple...
Grating Spectrophotometer with UV SCI/SCE Bluetooth
YR053426 // YR053428 Series grating spectrophotometer adopts bluetooth and USB dual modes for wider use...
Our best-selling spectrophotometer 🏆
- Dual optical path, dual beam optical system, dual detector, high performance imported grid, lower parasitic light, greater stability, reliability, more accurate analysis
- Equipped with 10" touch screen, built-in 32G memory, Bluetooth network support
- Dynamic functions, DNA/protein testing, multiple wavelength testing and data printing
- Adopt the suspension design of the optical system, the entire optical path is set independently on the deformation-free base of aluminum 16 mm thick
- Vibration from the outside has no impact on the optical system, greatly improving the stability and reliability of the instrument
| Model | YR01862 | YR01862-1 | |
| Wave range | 190-1100 nm | ||
| Spectral Bandwidth. | 1.8nm | 0.5, 1.0 2.0, 4.0 nm | |
| Accuracy of the wavelength | ± 0.1nm (D2 656.1nm), ± 0.3nm in full range | ||
| Light gone | 0.03% T @ 220 nm, 360 nm | ||
| Wavelength-repeatability | ≤0.1nm | ||
| Photometric accuracy | 士 0.2% T (0-100% T) ± 0.002Abs (0-0.5Abs) ± 0.004Abs (0.5-1.0Abs) |
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| Photometric repeatability | ≤0.1% T (0-100% T) 0.001Abs (0-0.5Abs) 0.002Abs (0.5-1.0Abs) |
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| Stability | 士 0.0004A/[email protected] | ||
| Photometric Range | 0-200% T, -0.3-3.0A, 0-999C | ||
| Planitude of the baseline | 士 0.001A | ||
| Noise | 士 0.0004A | ||
| Monitor | 800*480 Touch Screen | ||
| Data-out port | USB | ||
| Print Port | USB port | ||
| Source of light | Tungsten lamp and deuterium lamp | ||
| power requirements | AC 220V / 50Hz or AC 110v / 60Hz | ||
| Detector | Silicon photodiode | ||
| Dimension | 630 * 430 * 210 nm | ||
| Weight | 28KG | ||
Analysis of the best spectrophotometers for your laboratory
Spectrophotometers: What are the main brands?
A spectrophotometer is a laboratory instrument used to measure the absorbance of a sample, depending on the...
What is a UV-VIS spectrophotometer?
UV-VIS spectroscopy is an analytical technique that measures the amount of discrete wavelengths of UV or visible light...
When to calibrate my spectrophotometer?
The calibration process, according to instructions for use, should be performed at most every 14 days. Our recommendation...
What are the uses of a spectrophotometer?
The spectrophotometer quantifies the concentration of a specific substance that is present in a sample...
Catalog of spectrophotometer models on offer
Spectrophotometer videos in operation
In this section you can find our spectrophotometers in operation, packed, receiving service, etc.
How does a spectrophotometer work?
This short animation shows the inner workings of a spectrophotometer. Practice using a virtual spectrophotometer.
So is as works a spectrophotometer. One lamp provides the source of light. The beam of light hit the grid of diffraction, that works as a prism and separate the light in your lengths of wave components.
The grid be tour of shape that alone one length of wave specific of the light arrives a the slit of exit. Then the light interacts with the sample. A split of east point, the detector measure the transmittance and absorbance of the sample.
Kalstein spectrophotometer
Spectrophotometers, in general, are very specialized equipment. Its conservation depends largely on the way of installation and use. The surrounding environment and the quality of electricity services are vital factors in enabling equipment to provide services in accordance with the specifications for which it was manufactured.
Maintenance procedures that may require varying complexity, from thorough cleaning of their components to specialized procedures, shall be performed only by trained technicians or engineers with the technical information developed by the manufacturers.
blanco" ] blanco" ]Frequently asked questions about spectrophotometers from our customers
How to know the prices of spectrophotometer?
To know the price of spectrophotometers we invite you to send us an email with your request through the contact form.
What are the delivery times of spectrophotometers?
The delivery time of your Kalstein product will depend on the following:
- If the equipment of your interest is in stock or if on the contrary it should be manufactured.
- The type of freight you have chosen may be; air or sea.
- Equipment in stock:
- Delivery Time ( Aereo ): 15 -30 days .
- Delivery Time ( Maritime ): 45-60 days .
- Equipment not in stock:
- Delivery Time ( Aereo ): 30 -60 days .
- Delivery Time ( Maritime ): 60-90 days .
How to make a purchase of spectrophotometers?
Can realize your purchase through :
- By email: sales@kalstein.eu
- By phone: +33 (0) 1 78 95 87 02
- Trade electronic : Through from Kalstein's official website in your country .
How does the warranty work?
All Kalstein equipment has a 1-year warranty against manufacturing defects. The warranty does not cover damage caused by a poor installation or operation by the user, transportation defects or uses other than those specified by the manufacturer. Warranty excludes electrical or consumable parts. For more information visit our "terms and conditions " by clicking HERE
At Kalstein we provide our customers with inductions and technical support through new online methods. You can visit our inductions, technical assistance and guidance videos provided by a Kalstein team through our channel Youtube (Kalstein English).
Can I request a quote online?
Of course, you can request a quote for the Kalstein team of your interest, directly from our website officer. U Once you have identified your preferred model, make click HERE
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