Microscopes are essential equipment in any laboratory, some are very simple while others are more complex equipment; however, they are all made up of many parts that require proper maintenance, since they represent a large investment.
The first great advances in science and in particular in the biological sciences are due in part to the invention of the optical microscope, when at the end of the seventeenth century Anton van Leeuwenhoek, carving lenses, was able to appreciate the world that due to its small size was impossible see with the naked eye: the microscopic world.
Fortunately, years later, thanks to the invention of the optical microscope, man was able to have evidence of the great world that existed beyond the lenses and thus discover an inorganic universe, such as table salt crystals or oxalate salts that They are found in the urine and whose accumulation is the cause of kidney stones. Likewise, he was able to observe the slow movements of an intestinal parasite, the amoeba, which also helped to remove the blindfold of obscurantism and thus take the first steps in modern science.
Currently there are different types of microscopes, the luminous ones are those that use visible light in order to make a specimen observable. These include the simple light microscope and the compound light microscope.
Is the most used for the observation of colored smears, to examine morphological characteristics and the mobility of organisms in fresh preparations...
Shades the tones from light gray to very dark. It is based on the fact that the light waves that pass through transparent objects, such as cells, emerge in different phases, depending on the properties of the materials they pass through.
The same light microscope is used, using a condenser whose numerical aperture is greater than that of the objective, thus blocking direct light rays just as it deflects light from a mirror to the side of the condenser at an oblique angle.
Is based on the principle of removal of incident illumination by selective absorption, transmission of the light absorbed by the sample and re-emitted with different wavelengths.
The objective of a microscope is the most important and complex element of the light microscope. It is important because the objective is where most of the magnification provided by the microscope occurs. Its complexity lies in the fact that to fulfill its function you need different lenses of high quality and precision.
The microscope is an instrument that allows to observe objects that are not perceptible to the human eye. This is achieved by an optical system ...
Among the most important routines for maintaining a microscope in proper operating condition are the following:
The microscope is an instrument that allows to observe objects that are not perceptible to the human eye. This is achieved by an optical system ...
High-powered microscope is often unable to provide excellent quality images due to improper use of light sources, which generally leads to inadequate illumination of the object. You must take into account that any correctly illuminated sample must be free of glare and the light must be scattered evenly in the visual field.
Phase contrast microscopy was invented by the Dutch physicist Frits Zernike in 1932. This great invention earned him the Nobel Prize in Physics in 1953. The main difficulty in observing living cells was that they are practically transparent. For this reason, if they were looked at through a conventional microscope with transmitted light, it was very difficult or even impossible to observe their microscopic details and structures. The usual solution to this problem was to use staining techniques.
The problem in the use of dyes is that their addition is often incompatible with the life of the cells. For this reason, although they are suitable for observing dead cells, their use is often limited when the cells are to be kept alive.
For this type of observations, the phase contrast microscope was created. This microscope manipulates light in such a way that it is possible to increase the contrast of the observed sample. In this way it is possible to observe structures that are invisible through a conventional microscope.
