Product Description
The YR06248 and YR06249 models of the real-time quantitative PCR system are designed to provide unmatched precision and efficiency in molecular biology applications. These systems boast a comprehensive array of features designed to enhance real-time PCR analysis. With a sample capacity that accommodates up to 96 individual tubes and various PCR plate configurations, these systems offer versatility and adaptability for various research needs.
Both models utilize a Peltier-based heating and cooling method, ensuring a rapid maximum ramping rate of 6.0°C/s and maintaining temperature uniformity at ±0.2°C at 60°C and ±0.3°C at 95°C. The systems cover a wide temperature range from 4 to 100°C with an impressive accuracy of ±0.2°C, offering reliable thermal control critical for PCR precision.
Enhanced optical detection is achieved through the integration of high-efficiency single-color LEDs and sensitive SiPMT detectors. The systems support a broad spectrum of excitation and emission wavelengths, from 455-650nm and 510-750nm respectively, providing flexibility in experimental design. Furthermore, the detection channels range from 4 to 5, supporting multiplexing capabilities up to 4 or 5 targets, depending on the model, enhancing their utility in complex assays.
| Specification | YR06248 | YR06249 |
|---|---|---|
| Sample capacity |
|
|
| Sample volume | 10-50 μL | |
| Heating and Cooling method | Peltier | |
| Maximum ramping rate | 6.0°C/s | |
| Temperature range | 4-100 °C | |
| Temperature accuracy | ± 0.2°C | |
| Temperature uniformity | ±0.2°C@60°C ±0.3°C@95°C | |
| Optical Detecting System | ||
| Excitation light | 4pcs high-efficiency single color LED | 5pcs high-efficiency single color LED |
| Detector | SiPMT | |
| Detection method | Time-resolved real-time scanning | |
| Excitation/Emission wavelengths | 455-650nm | 510-750nm |
| Detection Channels | 4 (optional 5 channels） | |
| Supported dyes | FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ, (Optional- Cy5.5 for 5-channel model) | |
| Multiplexing | Up to 4 targets (optional 5 targets for 5-channel model) | |
| Sensitivity | 1 copy gene | |
| Resolution | 1.33-fold copies difference in single-plex reaction | |
| Dynamic range | 10 orders of magnitude | |
Market Price
The real-time quantitative PCR systems, YR06248 and YR06249, fall within a competitive price range in the market, generally ranging from $22,000 to $23,800 USD. This pricing reflects the high-quality design and advanced technological integration these systems offer, ensuring a valuable investment for laboratories seeking premium performance.
Frequently Asked Questions
What is the sample capacity of these models?
The YR06248 and YR06249 support up to 96 samples with various configurations, including individual tubes, strips, and PCR plates.
Are these systems compatible with multiplexing?
Yes, both systems support multiplexing up to 4 targets, with an option for 5 targets in the 5-channel configuration.
How accurate is the temperature control?
The systems maintain a temperature accuracy of ±0.2°C, crucial for high-precision experiments.
Advantages and Disadvantages
Advantages: The YR06248 and YR06249 PCR systems offer quick thermal cycling with a high ramping rate, precise temperature control, flexibility in sample handling, and broad dye support for varied experimental designs.
Disadvantages: While their advanced features justify the price, the investment is substantial, which may not be feasible for smaller laboratories with constrained budgets.
Field Application
The YR06248 and YR06249 real-time PCR systems are extensively used in genomics, diagnostics, and biotechnological research. Their high sensitivity and multiplexing capabilities enable detailed analysis of genetic material and pathogen detection, providing pivotal data in clinical and research settings.
The systems’ robust design ensures reliable performance even with extensive use, making them ideal for environments requiring consistent, high-throughput PCR testing.
Recommendations
To maximize the lifespan and efficiency of the YR06248 and YR06249 systems, regular maintenance checks and calibration are recommended. Utilizing high-quality reagents and adhering to manufacturer guidelines can further ensure accurate results and optimal system performance.
Consider integrating these PCR systems with Kalstein’s comprehensive platform for seamless laboratory operations and support. For those seeking an upgrade in their PCR capabilities, obtaining a quote through Kalstein Plus could be the next step in enhancing your research potential.
If you are looking for a blend of innovation and quality, you have come to the right place. At Kalstein we offer you the luxury of browsing our exclusive catalog of laboratory equipment. We manufacture each piece with a level of excellence. Our online purchasing channels, intuitive and agile, are designed for your convenience, ensuring the most friendly prices. Do not hesitate any longer, we bring science to life, it is time to become part of our community. https://kalstein.net/en/product/real-time-quantitative-pcr-system-yr06248-yr06249/.
