Description of the Product
The PCR Thermal Cycler YR01868 is a state-of-the-art laboratory instrument designed for high throughput and precision in nucleic acid amplification. With a processing capacity of up to 48 samples, this cycler is equipped with two fluorescence channels, significantly broadening its application range in sophisticated laboratory environments. The innovative LED light source ensures high brightness and extended lifespan with minimal maintenance, enhancing both efficiency and ease of use.
This sophisticated device is compatible with a range of dyes, including FAM, SYBR Green I, SYTO 9, Eva Green, LC Green in Channel 1, and HEX, VIC, TET, JOE in Channel 2. The optical system leverages photodiodes (PDs) as detectors, covering an excitation range from 470 nm to 638 nm and a detection range from 525 nm to 685 nm. Equipped with a Peltier heating method, the thermal cycler achieves a considerable heating rate of ≥8.0 ℃/s and a cooling rate of ≥6.2 ℃/s, ensuring rapid cycle completion.
| Model | YR01868 |
| Throughput | 48 samples |
| Fluorescence Channels | 2 |
| Fluorescence Scanning Time | 2s |
| Dye Compatibility | Channel 1: FAM, SYBR Green I , SYTO 9, Eva Green, LC Green |
| Channel 2: HEX, VIC, TET, JOE | |
| Light Source | High-brightness, long life and maintenance-free LED light source |
| Detector | Photodiodes (PDs) |
| Excitation Range | CH1: 470nm CH2:523nm CH3:570nm CH4:638nm |
| Detection Range | CH1:525nm CH2:564nm CH3:610nm CH4:685nm |
| Fluorescence Dynamic Range | Adjustable |
| Sample Dynamic Range | From 101 to 1010 copies |
| Heating Method | Peltier |
| Heating Rate | ≥8.0 ℃/s |
| Cooling Rate | ≥6.2 ℃/s |
| Temperature Accuracy | ≤0.1 ℃ |
| Gradient Range | 1℃-40 ℃ |
| Gradient Block | 8 row |
| Sample Testing Linearity and Repeatability | Linear correlation: /r/ ≥ 0.999 |
| Repeatability: cycle threshold (Ct) value CV≤0.5% | |
| Control Modes | Model1: 7-inch touch screen |
| Mode 2: PC direct control | |
| Data Storage and Transmission | Upload and download through USB disk. 1000 results can be stored in machine |
| Key Applications | Relative quantification, absolute quantification, melting curve analysis, SNP analysis |
| Operating System for PC | Win7/Win10/Win11 |
| Power Supply and Power Consumption | AC 100-240V, 50-60HZ; 600VA |
| Weight | 11 Kg (net) |
| Instrument Dimension | 260*400*260mm (W*L*H) |
| Suitable Consumables | 0.2mL clear non-skirt 48-WELL plates, 8-tube strips, single tubes, etc. |
Market Price
The PCR Thermal Cycler YR01868, with its advanced features and robust performance capabilities, is positioned in the market with a price ranging from $15,500 to $16,500. This pricing reflects the high-quality components and cutting-edge technology embedded in the device, ensuring users receive excellent value for their investment.
As the demand for efficient and reliable thermal cyclers continues to rise, laboratories prioritize acquiring equipment that combines precision with versatility. For those interested in purchasing this high-caliber cycler, you can seamlessly request a quote or generate an automatic one through the Kalstein Plus platform, making the purchase process effortless and efficient.
Frequently Asked Questions
What sets the YR01868 model apart from other thermal cyclers?
The YR01868 model is distinguished by its dual fluorescence channels and fast scanning time, making it suitable for a range of complex analyses such as SNP analysis and quantification tasks.
Can this model handle various PCR protocols?
Yes, the YR01868 is compatible with advanced PCR protocols including thermal gradients PCR, long PCR, and Touch Down PCR, offering considerable flexibility in experimental designs.
How is data managed and stored on the YR01868?
The unit can store up to 1000 results internally and supports data upload and download via USB, facilitating seamless data handling and management.
Advantages and Disadvantages
Advantages: The PCR Thermal Cycler YR01868 boasts a high throughput of 48 samples per run, which significantly enhances laboratory productivity. Its adjustable fluorescence and sample dynamic ranges allow for customized application as per the experimental needs. Moreover, the precise temperature control with a gradient range of 1℃ to 40 ℃ ensures highly accurate results. The device’s intuitive control modes, which include a 7-inch touch screen and PC direct control, provide versatility in operation.
Disadvantages: Despite its numerous strengths, the YR01868 may pose higher operational costs in terms of initial investment. Also, its compact design could present limitations for larger labs requiring higher throughput instruments.
Field Use
In practical settings, the PCR Thermal Cycler YR01868 is predominantly used for DNA amplification in genetic research, clinical diagnostics, and forensic laboratories. Its precise thermal control and quick cycling capabilities are ideal for time-sensitive applications where reliability and accuracy are paramount. Researchers and lab technicians widely use it for multiplex PCR assays, which require different temperatures across tests.
The model also supports comprehensive analyses, including relative and absolute quantification, using advanced fluorescence technology. This enables detailed studies of gene expression and polymorphism, crucial in medical research and treatment evaluation.
Recommendations
For optimal performance of the PCR Thermal Cycler YR01868, regular maintenance and proper handling according to the manufacturer’s guidelines are essential. Users should ensure the instrument is cleaned regularly, particularly the optical components, to maintain precise readings. It’s advisable to calibrate the equipment periodically, especially when working with new sample types or after long periods of inactivity.
Additionally, it is recommended to familiarize oneself with the software functions to harness the cycler’s full potential effectively. Ensuring the correct consumables, such as 0.2mL clear non-skirt 48-WELL plates, are used will also help maintain operational efficiency.
If you are looking for a fusion of innovation and quality, you have come to the right place. At Kalstein, we offer you the luxury to explore our exclusive catalog of laboratory equipment. We manufacture each equipment with a level of excellence. Our intuitive and agile online shopping channels are designed for your convenience, ensuring the most friendly prices. Do not hesitate any longer, we bring science to life, it is time to be part of our community. https://kalstein.net/en/product/pcr-thermal-cyclers-yr01868/.
