Product Description
The Kalstein YR01869 thermal cycler is a cutting-edge instrument designed to meet the rigorous demands of modern molecular biology laboratories. With a throughput capacity of 96 samples and six fluorescence channels, this thermal cycler allows for efficient and precise DNA amplification. The YR01869 model is equipped with a high-brightness, long-life LED light source, providing excitation from the top, and utilizes photodiodes for top scanning detection. Its dye compatibility spans various channels, including FAM, SYBR Green I, HEX, VIC, ROX, and others, supporting a broad range of applications.
The YR01869’s thermal performance is enhanced by a Peltier heating method with rates exceeding 6.1 °C/s for heating and 5.0 °C/s for cooling. Temperature uniformity and accuracy are maintained at ±0.1 °C and ≤0.1 °C, respectively. Additionally, the device supports specialized temperature protocols such as thermal gradients, long PCR, and Touch Down PCR, facilitating versatile experimental designs. Control options include a 10.4-inch touchscreen or PC direct control, ensuring user-friendly operation. For data management, the YR01869 offers USB-based data storage and transmission for 1000 results, along with customizable experiment reports.
| Model | YR01869 |
| Throughput | ene-96 |
| Fluorescence Channels | 6 |
| Fluorescence Scanning Time | 7s |
| Dye Compatibility | Channel 1: FAM, SYBR Green I , SYTO 9, Eva Green, LC Green |
| Channel 2: HEX, VIC, TET, JOE | |
| Channel 3: ROX, Texas Red | |
| Channel 4: Cy5 | |
| Channel 5: Alexa Fluor 680 | |
| Channel 6: FRET | |
| Optical System | |
| Light Source | High-brightness, long life and maintenance-free LED light source, excitation from the top |
| Detector | Photodiodes (PDs), top scanning |
| Excitation Range | CH1: 465nm CH2:527nm CH3:580nm CH4:632nm CH5:680nm CH6:465nm |
| Detection Range | CH1: 510nm CH2:563nm CH3:616nm CH4:664nm CH5:730nm CH6:616nm |
| Fluorescence Dynamic Range | Adjustable |
| Sample Dynamic Range | From 101 to 1010copies |
| Thermal Black | |
| Heating Method | Peltier |
| Heating Rate | >6.1 ℃/s |
| Cooling Rate | >5.0 ℃/s |
| Temperature Uniformity | ±0.1 ℃ |
| Temperature Accuracy | ≤0.1 ℃ |
| Gradient Range | 1℃-40 ℃ |
| Gradient Block | 12 row |
| Special Temperature Protocol | Thermal gradients PCR, long PCR, Touch Down PCR |
| Sample Testing Linearity and Repeatability | Linear correlation: /r/ ≥ 0.999 |
| Repeatability: cycle threshold (Ct) value CV≤0 0.5% | |
| Software Functions | |
| Control Modes | Model 1: 10.4 inch touch screen |
| Mode 2: PC direct control | |
| Power Failure Protection | Automatically start running experiments after power supply, no need to wait PC software |
| Data Storage and Transmission | Upload and download through USB disk. 1000 results can be stored in machine |
| Reporting Function | Templates reserved; customized experiment report |
| Key Applications | Relative quantification, absolute quantification, melting curve analysis ,SNP analysis |
| Operating System for PC | Win7/Win10/Win11 |
| Power Supply and Power Consumption | AC 100-240V,50-60HZ, 900VA |
| Weight | 30 Kg (net) |
| Instrument Dimension | 555*475*484mm (W*L*H) |
| Suitable Consumables | 0.2mL 96-well plates, 8-tube strips, single tubes(clear, frosted and white). |
Market Price
The price range for the YR01869 thermal cycler typically varies from approximately $28,500 USD to $29,500 USD, depending on the supplier and any additional features or services included. This average pricing positions it competitively in the market, offering excellent value given its comprehensive capabilities and advanced technology.
Frequently Asked Questions
What are the primary uses of the YR01869 thermal cycler?
The YR01869 is primarily used for DNA amplification in molecular biology applications, including relative and absolute quantification, melting curve analysis, and SNP analysis.
What kind of samples can the thermal cycler handle?
This unit is compatible with 0.2mL 96-well plates, 8-tube strips, and single tubes, whether they are clear, frosted, or white.
Does the YR01869 have built-in data storage?
Yes, the thermal cycler can store up to 1000 results internally and supports USB-based data transfer for easy uploading and downloading of experimental data.
Advantages and Disadvantages
The YR01869 thermal cycler boasts numerous advantages, such as high-throughput capabilities, multiple fluorescence channels, and precise temperature control. These features ensure excellent reproducibility and flexibility in experimental design. Additionally, the robust LED light source and top scanning photodiodes enhance accuracy and reduce maintenance needs.
However, its sophisticated design and advanced features might present a learning curve for inexperienced users. Additionally, laboratories with limited budgets might find the initial investment significant. Despite these considerations, the YR01869 is a valuable addition to any lab seeking performance and versatility.
Usage in the Field
In a typical laboratory setting, the YR01869 thermal cycler is utilized for a variety of applications, such as PCR amplification, genotyping, and cloning experiments. Its flexible control options enable seamless integration into lab workflows, optimizing time and resource management. Researchers can design experiments with different protocols, thanks to its adaptable thermal and fluorescence dynamics.
Moreover, the cycler’s compatibility with a range of dyes and detection channels allows for both qualitative and quantitative analyses, facilitating detailed investigations into genetic material. The instrument’s robust features and intuitive interface make it valuable in both research and clinical labs.
Recommendations
To maximize the benefits of the YR01869 thermal cycler, users should familiarize themselves with its control modes and software functionalities. Regular maintenance and calibration can enhance the instrument’s longevity and performance. Additionally, leveraging Kalstein Plus for quote generation can ensure cost-effective procurement and access to the latest updates and product support.
Engaging with Kalstein’s dedicated support team can provide valuable insights into optimizing protocols and addressing any technical challenges. By exploring its comprehensive features, users can expand their research possibilities and drive innovation in their respective fields.
If you are seeking a fusion of innovation and quality, you have come to the right place. At Kalstein, we offer you the luxury of exploring our exclusive catalog of laboratory equipment. We manufacture each piece of equipment with a level of excellence. Our online purchase channels, intuitive and agile, are designed for your convenience, ensuring the most friendly prices. Don’t hesitate any longer, we bring science to life, it’s time to become part of our community. https://kalstein.net/en/product/thermal-cyclers-yr01869/.
