Product Description
The YR01869 Thermal Cycler is a state-of-the-art device designed for high-efficiency PCR processes, renowned for its robust technical specifications and user-friendly interface. With a throughput capacity of up to 96 samples, this model is ideal for labs that demand precision and speed in their thermal cycling tasks.
This thermal cycler is equipped with six fluorescence channels, making it compatible with a diverse range of dyes, ensuring versatility in experiments. The fluorescence scanning time is an impressive 7 seconds, optimizing time without compromising the quality of results. The advanced dye compatibility includes Channel 1: FAM, SYBR Green I, SYTO 9, Eva Green, LC Green; Channel 2: HEX, VIC, TET, JOE; Channel 3: ROX, Texas Red; to Channel 4: Cy5, Channel 5: Alexa Fluor 680, and Channel 6 FRET, facilitating a wide array of applications.
The optical system features a high-brightness, long-life LED light source that ensures maintenance-free operation, with top-excitation enhancing the performance. The photodiodes for the detector, along with a broad excitation and detection range per channel, elevate this instrument’s reliability in providing consistent and precise results.
Its thermal capabilities are supported by the Peltier heating method, boasting a heating rate of over 6.1 ℃/s and a cooling rate exceeding 5.0 ℃/s, paired with excellent temperature uniformity of ±0.1 ℃ and high temperature accuracy of ≤0.1 ℃. These features contribute to a gradient range of 1℃-40℃ spread over a 12-row gradient block.
The YR01869’s control can be handled via a 10.4-inch touchscreen or directly through a PC, with built-in power failure protection ensuring seamless resumption of experiments post-outage. The ability to store up to 1000 results, data upload and download through USB, and customizable report templates further streamline laboratory workflows.
| Model | YR01869 |
| Throughput | 01-96 |
| Fluorescence Channels | 6 |
| Fluorescence Scanning Time | 7s |
| Dye Compatibility | Channel 1: FAM, SYBR Green I, SYTO 9, Eva Green, LC Green |
| Channel 2: HEX, VIC, TET, JOE | |
| Channel 3: ROX, Texas Red | |
| Channel 4: Cy5 | |
| Channel 5: Alexa Fluor 680 | |
| Channel 6: FRET | |
| Optical System | |
| Light Source | High-brightness, long life and maintenance-free LED light source, excitation from the top |
| Detector | Photodiodes (PDs), top scanning |
| Excitation Range | CH1: 465nm CH2:527nm CH3:580nm CH4:632nm CH5:680nm CH6:465nm |
| Detection Range | CH1: 510nm CH2:563nm CH3:616nm CH4:664nm CH5:730nm CH6:616nm |
| Fluorescence Dynamic Range | Adjustable |
| Sample Dynamic Range | From 101 to 1010 copies |
| Thermal Block | |
| Heating Method | Peltier |
| Heating Rate | >6.1 ℃/s |
| Cooling Rate | >5.0 ℃/s |
| Temperature Uniformity | ±0.1 ℃ |
| Temperature Accuracy | ≤0.1 ℃ |
| Gradient Range | 1℃-40 ℃ |
| Gradient Block | 12 row |
| Special Temperature Protocol | Thermal gradients PCR, long PCR, Touch Down PCR |
| Sample Testing Linearity and Repeatability | Linear correlation: /r/ ≥ 0.999 |
| Repeatability: cycle threshold (Ct) value CV≤0 0.5% | |
| Software Functions | |
| Control Modes | Mode 1: 10.4 inch touch screen |
| Mode 2: PC direct control | |
| Power Failure Protection | Automatically start running experiments after power supply, no need to wait for PC software |
| Data Storage and Transmission | Upload and download through USB disk. 1000 results can be stored in the machine |
| Reporting Function | Templates reserved; customized experiment report |
| Key Applications | Relative quantification, absolute quantification, melting curve analysis, SNP analysis |
| Others | |
| Operating System for PC | Win7/Win10/Win11 |
| Power Supply and Power Consumption | AC 100-240V,50-60HZ, 900VA |
| Weight | 30 Kg (net) |
| Instrument Dimension | 555*475*484mm (W*L*H) |
| Suitable Consumables | 0.2mL 96-well plates, 8-tube strips, single tubes (clear, frosted, and white). |
Market Price
The YR01869 Thermal Cycler positions itself competitively within the market for this category of advanced laboratory equipment. The typical market pricing for such a device averages between $29,000 and $30,500, reflecting its advanced technological capabilities and robust features designed for top-tier laboratory use.
The investment in such high-quality equipment ensures laboratories benefit from superior performance, longevity, and a wide range of functionality that supports complex experimental processes. The pricing structure aligns with the added value the device brings to enhancing scientific accuracy and efficiency.
Frequently Asked Questions
Is the YR01869 Thermal Cycler compatible with different types of assays?
Yes, the YR01869 Thermal Cycler is compatible with a wide range of assays due to its multiple fluorescence channels and broad dye compatibility.
What are the main control options available for this cycler?
The device can be controlled through a user-friendly 10.4-inch touchscreen or directly by connecting to a PC, providing flexibility in operation.
Does it offer any power failure protection features?
Yes, it includes automatic power failure protection, allowing experiments to resume without the need for intervention, which is important for maintaining integrity in your experimental results.
Advantages and Disadvantages
Advantages:
The YR01869 Thermal Cycler presents multiple advantages such as rapid heating and cooling rates, superior temperature uniformity, and remarkable accuracy. It handles high throughput volumes effectively, supports detailed experiment customization through its robust software functions, and offers extensive compatibility with a range of dyes.
Moreover, its intuitive interface and dual-mode control (touchscreen and PC) enhance ease of use, while the ability to store a substantial volume of data supports efficient laboratory management.
Disadvantages:
A potential drawback could be its cost, which may be prohibitive for smaller labs with limited budgets. Also, the instrument’s physical size and weight may require dedicated space within the lab.
Product Usage in the Field
The YR01869 Thermal Cycler is invaluable in a variety of laboratory settings, especially those engaging with high-throughput DNA amplification and various PCR applications. Its high precision in controlling thermal cycles makes it perfect for environments requiring stringent control for accurate sample processing.
This product finds extensive application in genetic research, medical diagnostics, and educational labs, where experiments such as quantitative PCR and SNP analysis are frequently performed. Its reliability and adaptability in handling various consumables further bolster its practicality in diverse research fields.
Recommendations
For optimal performance of the YR01869 Thermal Cycler, it is recommended to ensure adequate training for operational staff to utilize both control options effectively. Regular maintenance and adhering to manufacturer guidelines for calibrations can prolong the lifespan and efficiency of the instrument.
While planning the lab setup, consider allocating sufficient space to accommodate the cycler’s dimensions and ensure stable power supply connections. For labs handling various assay types, maintaining an inventory of compatible dyes and consumables is advisable for smooth operational workflows.
Considering the technical sophistication of this thermal cycler, obtaining a quote or exploring Kalstein’s platform for additional customization options and pricing can be a proactive step to ensure the equipment meets your specific lab requirements.
If you’re looking for a fusion of innovation and quality, you’ve come to the right place. At Kalstein, we offer the luxury of exploring our exclusive catalog of laboratory equipment. We manufacture each piece with a standard of excellence. Our intuitive and agile online purchasing channels are designed for your convenience, ensuring the most friendly prices. Do not hesitate anymore, we give life to science, it’s time to become part of our community. https://kalstein.net/en/product/thermal-cyclers-yr01869/.
