Product Description
The YR01869 thermal cycler is a state-of-the-art laboratory device designed for precision and efficiency in PCR processes. Its ability to handle up to 96 samples simultaneously makes it ideal for high-throughput applications. Equipped with six fluorescence channels, the YR01869 is compatible with a wide range of dyes, including FAM, SYBR Green I, HEX, VIC, and more, offering unparalleled versatility in experimental design.
This thermal cycler boasts a rapid fluorescence scanning time of just 7 seconds and uses a high-brightness, maintenance-free LED light source for excitation from the top, partnered with photodiode detectors for top-scanning detection. The device covers an extensive excitation range from 465nm to 680nm, and a detection range from 510nm to 730nm, ensuring accurate and reliable data collection. Its heated lid ensures precise temperature control, with a heating rate exceeding 6.1 ºC/s and a cooling rate of more than 5 ºC/s, maintaining uniformity and accuracy to within ±0.1 ºC.
Incorporating dynamic thermal gradients, the YR01869 supports complex protocols such as long PCR and touchdown PCR. The user-friendly control options, including a 10.4-inch touchscreen and PC direct control, provide flexibility in operation. Moreover, it includes smart features like automatic resume post power failure, USB data storage, and customizable experiment reports for seamless data management.
| Model | YR01869 |
| Throughput | 96 |
| Fluorescence Channels | 6 |
| Fluorescence Scanning Time | 7s |
| Dye Compatibility | Channel 1: FAM, SYBR Green I, SYTO 9, Eva Green, LC Green |
| Channel 2: HEX, VIC, TET, JOE | |
| Channel 3: ROX, Texas Red | |
| Channel 4: Cy5 | |
| Channel 5: Alexa Fluor 680 | |
| Channel 6: FRET | |
| Optical System | |
| Light Source | High-brightness, long life and maintenance-free LED light source, excitation from the top |
| Detector | Photodiodes (PDs), top scanning |
| Excitation Range | CH1: 465nm CH2: 527nm CH3: 580nm CH4: 632nm CH5: 680nm CH6: 465nm |
| Detection Range | CH1: 510nm CH2: 563nm CH3: 616nm CH4: 664nm CH5: 730nm CH6: 616nm |
| Fluorescence Dynamic Range | Adjustable |
| Sample Dynamic Range | From 10¹ to 10¹º copies |
| Thermal Black | |
| Heating Method | Peltier |
| Heating Rate | >6.1 ºC/s |
| Cooling Rate | >5.0 ºC/s |
| Temperature Uniformity | ±0.1 ºC |
| Temperature Accuracy | ≤0.1 ºC |
| Gradient Range | 1 ºC-40 ºC |
| Gradient Block | 12 row |
| Special Temperature Protocol | Thermal gradients PCR, long PCR, Touch Down PCR |
| Sample Testing Linearity and Repeatability | Linear correlation: /r/ ≥ 0.999 |
| Repeatability: cycle threshold (Ct) value CV≤0 0.5% | |
| Software Functions | |
| Control Modes | Model 1: 10.4 inch touch screen |
| Mode 2: PC direct control | |
| Power Failure Protection | Automatically start running experiments after power supply, no need to wait PC software |
| Data Storage and Transmission | Upload and download through USB disk. 1000 results can be stored in machine |
| Reporting Function | Templates reserved; customized experiment report |
| Key Applications | Relative quantification, absolute quantification, melting curve analysis, SNP analysis |
| Others | |
| Operating System for PC | Win7/Win10/Win11 |
| Power Supply and Power Consumption | AC 100-240V, 50-60HZ, 900VA |
| Weight | 30 Kg (net) |
| Instrument Dimension | 555*475*484mm (W*L*H) |
| Suitable Consumables | 0.2mL 96-well plates, 8-tube strips, single tubes (clear, frosted and white). |
Market Price
The YR01869 thermal cycler is positioned in the market as a top-tier laboratory apparatus, aligning closely with a price range typically ranging from $28,700 to $29,500. This range reflects the product’s advanced features and the precision engineering that it encapsulates, making it a worthwhile investment for laboratories focused on obtaining superior PCR results.
Pricing may vary slightly based on suppliers, shipping options, and potential accessories included. For accurate and customized pricing tailored to your laboratory’s needs, we recommend requesting a quote directly. This ensures you receive the most favorable price while exploring options for additional support or warranty extensions.
Frequently Asked Questions
What types of dyes can the YR01869 use?
The YR01869 is compatible with a range of dyes across its six channels, including FAM, SYBR Green I, HEX, VIC, ROX, Texas Red, Cy5, and Alexa Fluor 680, among others.
Does the thermal cycler come with software controls?
Yes, the YR01869 includes dual control modes: a 10.4-inch touch screen for easy on-device management and PC direct control for seamless integration with lab computer systems.
What is the warranty period for the YR01869 thermal cycler?
The warranty details can vary, so it is advisable to confirm this with the supplier upon purchase, but typically it includes coverage to ensure smooth and reliable performance over several years.
Advantages and Disadvantages
Advantages: The YR01869 thermal cycler provides efficient throughput with the ability to process 96 samples simultaneously, supported by fast scanning times and six fluorescence channels. Its robust build and advanced temperature control contribute to highly accurate PCR results, while the option for touch screen or PC control enhances user convenience.
Disadvantages: As a highly specialized piece of equipment, the YR01869 may require a learning curve for optimal operation, especially for those accustomed to older models. Additionally, its cost, reflective of its advanced capabilities, may necessitate budget considerations for some facilities.
Field Application
In practical settings, the YR01869 is indispensable for genomic research and clinical diagnostics, where precision in PCR is crucial. Its reliable performance under varied thermal protocols makes it a key asset in experiments ranging from basic amplification to complex genetic analysis. The ability to handle various consumables, including 96-well plates and tube strips, further expands its applicability across different experimental setups.
The device’s robust design ensures consistent performance in demanding environments, ensuring that researchers can trust their data across multiple cycles and variations. This utility makes the YR01869 a favored choice among professionals seeking excellence in thermal cycling.
Recommendations
For optimal results, it is recommended to regularly calibrate the YR01869 and stay current with software updates from the manufacturer. Using only compatible consumables will ensure the longevity and efficiency of the equipment’s operation. When initially setting up the cycler, careful adherence to the installation instructions will prevent operational hiccups and maintain warranty validity.
For those investing in the YR01869, participating in training sessions or workshops offered by Kalstein can maximize user proficiency and ensure comprehensive understanding of its capabilities. This proactivity helps in harnessing the full potential of the equipment, optimizing its utility in ongoing and future projects.
If you are looking for a fusion of innovation and quality, you have come to the right place. At Kalstein, we offer you the luxury of exploring our exclusive catalog of laboratory equipment. We manufacture each piece of equipment with a level of excellence. Our intuitive and fast online shopping channels are designed for your convenience, ensuring the friendliest prices. Do not hesitate any longer, we bring science to life, it is time to become part of our community. https://kalstein.net/en/product/thermal-cyclers-yr01869/.
