Product Description
The Real-Time Quantitative PCR Systems, models YR06248 and YR06249, represent cutting-edge technology in the field of molecular biology. These systems are designed to deliver precise, rapid, and reliable DNA analysis for various applications, ranging from research to diagnostics. The devices feature a robust sample capacity of 8*0.1mL PCR strip*12, 96*0.1mL individual tubes, or a 0.1mL PCR plate, allowing users flexibility and efficiency in handling diverse sample types.
Designed with a sample volume range of 10-50 μL, these systems use the Peltier heating and cooling method, ensuring consistent thermal control with a maximum ramping rate of 6.0°C/s. Temperature settings are both versatile and precise, with a range of 4-100°C, accuracy of ± 0.2°C, and uniformity maintained at ±0.2°C@60°C and ±0.3°C@95°C. These capabilities highlight the system’s alignment with the highest standards of quantitative PCR processes.
The optical detecting system is optimized for efficiency with models YR06248 featuring four high-efficiency single-color LEDs and YR06249 offering five. Both are equipped with a SiPMT detector and employ time-resolved real-time scanning, boosting their detection capabilities. The systems support a wide range of dyes including FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, and Cy5/LIZ, with an optional Cy5.5 for the five-channel model.
User flexibility is further enhanced by multiplexing that supports up to four targets, extendable to five in the five-channel model, and exhibits high sensitivity able to detect a single copy gene. The systems promise distinction with a resolution of 1.33-fold copy differences in single-plex reactions and a dynamic range spanning 10 orders of magnitude, making them exceptionally versatile for complex PCR experiments.
| Model | YR06248 | YR06249 |
|---|---|---|
| Sample capacity | 8*0.1mL PCR strip*12 96*0.1mL individual tube 0.1mL PCR Plate | |
| Sample volume | 10-50 μL | |
| Heating and Cooling method | Peltier | |
| Maximum ramping rate | 6.0° C/s | |
| Temperature range | 4-100 °C | |
| Temperature accuracy | ± 0.2°C | |
| Temperature uniformity | ±0.2°C@60°C ±0.3°C@95°C | |
| Excitation light | 4pcs high-efficiency single color LED | 5pcs high-efficiency single color LED |
| Detector | SiPMT | |
| Detection method | Time-resolved real-time scanning | |
| Excitation/Emission wavelengths | 455-650nm | 510-750nm |
| Detection Channels | 4 (optional 5 channels） | |
| Supported dyes | FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ, (Optional- Cy5.5 for 5-channel model) | |
| Multiplexing | Up to 4 targets (optional 5 targets for 5-channel model) | |
| Sensitivity | 1 copy gene | |
| Resolution | 1.33-fold copies difference in single-plex reaction | |
| Dynamic range | 10 orders of magnitude | |
Market Price
The market price for highly advanced Real-Time Quantitative PCR Systems like the YR06248 and YR06249 models generally falls within the range of $22,500 to $23,800 USD. Given their sophisticated features, the pricing is competitive in the landscape of modern laboratory equipment. It’s crucial that potential buyers consider the long-term value and technological benefits offered by these PCR systems when evaluating costs.
Frequently Asked Questions
What is the main difference between models YR06248 and YR06249?
The primary distinction lies in the optical detection system; YR06249 features an additional LED, allowing for broader excitation/emission wavelength coverage compared to YR06248, making it apt for more diverse applications.
How many samples can be processed simultaneously?
Both models accommodate up to 96 samples in 0.1mL individual tubes, offering substantial capacity for high-throughput projects.
What type of applications are these PCR systems suitable for?
These systems are ideal for quantitative and qualitative PCR-based experiments, including gene expression studies, genotyping, and pathogen detection, owing to their high sensitivity and precision.
Advantages and Disadvantages
Advantages:
- High throughput capability with a sample capacity accommodating various formats.
- Exceptional sensitivity down to a single gene copy, ensuring reliability in detection.
- Advanced temperature control mechanisms providing precise thermal cycling.
- Versatile optical system supporting a broad array of fluorescent dyes for multiplex assays.
- Dynamic range spanning 10 orders of magnitude, facilitating diverse experimental needs.
Disadvantages:
- The initial investment may be significant for smaller labs with limited budgets.
- The complexity of operation may require additional training for inexperienced users.
Product Use in the Field
In practical settings, the YR06248 and YR06249 PCR systems serve as critical tools in both academic research and clinical laboratories. They seamlessly integrate into workflows for real-time PCR analysis, expediting processes such as pathogen detection and genotyping. Their high sensitivity and multiplexing capabilities enable researchers to conduct comprehensive and conclusive analyses, crucial for accurate diagnostics and ground-breaking research.
Laboratories benefit from the systems’ robust construction and intuitive interface, reducing operational bottlenecks and enhancing analytical accuracy. By streamlining workflows, these PCR systems significantly impact productivity and scientific outcomes in both routine and cutting-edge research scenarios.
Recommendations
For optimal performance, it is recommended to ensure regular maintenance and calibration of the PCR systems to preserve accuracy and longevity. Users should adhere to the manufacturer’s guidelines regarding the use of compatible dyes and sample volumes to prevent errors and instrument wear. Routine software updates provided by Kalstein will enhance system functionality and security, ensuring users leverage the full spectrum of technological advancements available in these models.
It’s also advisable to participate in training sessions offered by Kalstein to maximize user proficiency and equipment utilization, ensuring that laboratory personnel are well-equipped to handle the complexities of modern PCR techniques.
If you are seeking a blend of innovation and quality, you have arrived at the right place. At Kalstein, we offer you the luxury to explore our exclusive catalog of laboratory equipment. We manufacture each equipment with a level of excellence. Our online purchase channels are intuitive and agile, designed for your convenience, ensuring the most friendly prices. Do not hesitate any longer, we bring science to life, it’s time to become part of our community. https://kalstein.net/en/product/real-time-quantitative-pcr-system-yr06248-yr06249/.
