Product Description
The YR01869 Thermal Cycler stands at the forefront of advanced thermocycling technology, offering unparalleled precision and adaptability for modern laboratory environments. Equipped to handle up to 96 samples simultaneously, this thermocycler is ideal for high-throughput applications. The inclusion of six fluorescence channels makes it possible to execute complex, multi-dye experiments with exceptional efficiency.
This state-of-the-art thermal cycler utilizes a high-brightness, long-life LED light source for excitation, ensuring dependable performance without the need for frequent maintenance. Coupled with top-scanning photodiodes, the optical system of the YR01869 guarantees precise measurements needed in critical applications such as relative and absolute quantification, as well as melting curve and SNP analyses.
A breakthrough in temperature management is achieved through Peltier heating, offering a heating rate of >6.1 ℃/s and a cooling rate of >5.0 ℃/s, maintaining uniformity and accuracy within ±0.1 ℃. This thermal stability ensures reliable results across various protocols such as gradient PCR, long PCR, and Touch Down PCR. The device also includes state-of-the-art control via a 10.4-inch touchscreen or direct PC control, providing flexible operation tailored to user preferences.
| Model | YR01869 |
| Throughput | 1-96 |
| Fluorescence Channels | 6 |
| Fluorescence Scanning Time | 7s |
| Dye Compatibility | Channel 1: FAM, SYBR Green I, SYTO 9, Eva Green, LC Green |
| Channel 2: HEX, VIC, TET, JOE | |
| Channel 3: ROX, Texas Red | |
| Channel 4: Cy5 | |
| Channel 5: Alexa Fluor 680 | |
| Channel 6: FRET | |
| Optical System | |
| Light Source | High-brightness, long life and maintenance-free LED light source, excitation from the top |
| Detector | Photodiodes (PDs), top scanning |
| Excitation Range | CH1: 465nm CH2: 527nm CH3: 580nm CH4: 632nm CH5: 680nm CH6: 465nm |
| Detection Range | CH1: 510nm CH2: 563nm CH3: 616nm CH4: 664nm CH5: 730nm CH6: 616nm |
| Fluorescence Dynamic Range | Adjustable |
| Sample Dynamic Range | From 101 to 1010 copies |
| Thermal Block | |
| Heating Method | Peltier |
| Heating Rate | >6.1 ℃/s |
| Cooling Rate | >5.0 ℃/s |
| Temperature Uniformity | ±0.1 ℃ |
| Temperature Accuracy | ≤0.1 ℃ |
| Gradient Range | 1℃-40 ℃ |
| Gradient Block | 12 row |
| Special Temperature Protocol | Thermal gradients PCR, long PCR, Touch Down PCR |
| Sample Testing Linearity and Repeatability | Linear correlation: /r/ ≥ 0.999 |
| Repeatability: cycle threshold (Ct) value CV≤0 0.5% | |
| Software Functions | |
| Control Modes | Model 1: 10.4 inch touch screen |
| Mode 2: PC direct control | |
| Power Failure Protection | Automatically start running experiments after power supply, no need to wait for PC software |
| Data Storage and Transmission | Upload and download through USB disk. 1000 results can be stored in machine |
| Reporting Function | Templates reserved; customized experiment report |
| Key Applications | Relative quantification, absolute quantification, melting curve analysis, SNP analysis |
| Others | |
| Operating System for PC | Win7/Win10/Win11 |
| Power Supply and Power Consumption | AC 100-240V, 50-60HZ, 900VA |
| Weight | 30 Kg (net) |
| Instrument Dimension | 555*475*484mm (W*L*H) |
| Suitable Consumables | 0.2mL 96-well plates, 8-tube strips, single tubes (clear, frosted, and white). |
Market Price
Considering the complex features and state-of-the-art specifications offered by the YR01869 Thermal Cycler, it typically falls within a premium price range in the market. The prices for thermocyclers with similar functionalities generally range from approximately $28,000 to $29,500. It is imperative for potential buyers to weigh the advantages offered by its sophisticated technology against their budget requirements to make informed purchasing decisions.
Frequently Asked Questions
What is the throughput capacity of this thermocycler?
The YR01869 has an impressive throughput capacity, capable of processing between one to 96 samples concurrently, making it suitable for high-volume laboratories.
Can the YR01869 handle multi-dye experiments?
Yes, it is equipped with six fluorescence channels to support complex multi-dye assays with precision and flexibility.
What control methods are available for this device?
The YR01869 offers dual control modes: a 10.4-inch touch screen for intuitive local operation and PC direct control for enhanced functionality.
Advantages and Disadvantages
Advantages:
- High throughput of up to 96 samples.
- Six channels for fluorescence detection, accommodating diverse dyes.
- Fast heating and cooling rates with precise thermal management.
- Broad application compatibility including SNP analysis and quantification tasks.
Disadvantages:
- As a high-end instrument, it comes at a premium price point.
- Comprehensive features may be underutilized in smaller labs with basic assay requirements.
Use of Product in the Field
The YR01869 Thermal Cycler is acclaimed for its robust application in various fields including molecular biology, clinical diagnostics, and genetic research. In practical settings, it is used for PCR amplifications in DNA analysis, marker-assisted selection in plant breeding, and high-throughput screening in biotech labs. Its ease of integration into existing workflows and compatibility with a wide range of consumables make it a highly favored choice among researchers and technicians.
Recommendations
For optimal performance, it is recommended to regularly calibrate and maintain the YR01869 Thermal Cycler according to the manufacturer’s guidelines. Ensuring the proper loading of samples and selecting appropriate PCR settings will maximize its efficiency. Additionally, leveraging the unit’s direct PC control can facilitate streamlined data management and sharing within collaborative research environments.
If you are searching for a fusion of innovation and quality, you have come to the right place. At Kalstein, we offer you the luxury of exploring our exclusive catalog of laboratory equipment. We manufacture each device with a level of excellence. Our intuitive and fast online purchasing channels are designed for your convenience, ensuring the most friendly prices. Don’t hesitate any longer, we bring science to life, it’s time to be a part of our community. https://kalstein.net/en/product/thermal-cyclers-yr01869/.
