Product Description
The YR06248 and YR06249 models are cutting-edge real-time quantitative PCR systems designed to facilitate high-precision genetic analysis in laboratory settings. These systems blend advanced technological features with user-friendly operations, making them ideal for research and diagnostic purposes. The key technical specifications are tailored to meet the evolving demands of molecular biology laboratories.
Both models offer a sample capacity of 96 individual 0.1mL tubes or one 0.1mL PCR plate, supporting a sample volume range of 10-50 μL. The heating and cooling mechanism employs a Peltier system, delivering a maximum ramping rate of 6.0° C/s within a temperature range of 4-100 °C. Precision is assured with a temperature accuracy of ±0.2°C and uniformity of ±0.2°C at 60°C and ±0.3°C at 95°C.
The optical detecting system in these models is also a highlight. The YR06248 is equipped with 4 high-efficiency single color LEDs, and the YR06249 with 5, providing excitation wavelengths between 455-650nm and 510-750nm respectively. Both systems utilize a SiPMT detector with time-resolved real-time scanning. They support multiplex PCR applications with up to 4 targets (and optional 5 targets for the YR06249) and an extensive dynamic range of 10 orders of magnitude.
The supported dyes include FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ, with an additional option for Cy5.5 in the 5-channel model. These systems ensure high sensitivity, capable of detecting a single gene copy, and provide exceptional resolution with a 1.33-fold copies difference in single-plex reactions.
| Model | YR06248 | YR06249 |
| Sample capacity | 8*0.1mL PCR strip*12, 96*0.1mL individual tube, 0.1mL PCR Plate | |
| Sample volume | 10-50 μL | |
| Heating and Cooling method | Peltier | |
| Maximum ramping rate | 6.0° C/s | |
| Temperature range | 4-100 °C | |
| Temperature accuracy | ± 0.2°C | |
| Temperature uniformity | ±0.2°C@60°C ±0.3°C@95°C | |
| Optical Detecting System | ||
| Excitation light | 4pcs high-efficiency single color LED | 5pcs high-efficiency single color LED |
| Detector | SiPMT | |
| Detection method | Time-resolved real-time scanning | |
| Excitation/Emission wavelengths | 455-650nm | 510-750nm |
| Detection Channels | 4 (optional 5 channels） | |
| Supported dyes | FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ，（Optional- Cy5.5 for 5-channel model） | |
| Multiplexing | Up to 4 targets (optional 5 targets for 5-channel model) | |
| Sensitivity | 1 copy gene | |
| Resolution | 1.33-fold copies difference in single-plex reaction | |
| Dynamic range | 10 orders of magnitude | |
Market Price
The current market price for these advanced PCR systems typically ranges between $22,500 and $23,500 USD. These systems present excellent value for money, considering the high-end features and technological sophistication they offer. Their cost-effectiveness is particularly notable for laboratories aiming to enhance their genetic analysis capabilities without a premium investment.
Frequently Asked Questions
What is the expected lifespan of the YR06248 and YR06249 PCR systems?
The expected lifespan of these PCR systems, with proper maintenance and care, can extend well beyond five to seven years.
Are these systems compatible with all standard PCR reagents?
Yes, both models are compatible with a wide range of standard PCR reagents, ensuring flexibility in experimental design and execution.
How can I ensure optimal performance?
Regular calibration, following maintenance guidelines, and using only recommended reagents will assure optimal performance and longevity.
Advantages and Disadvantages
Advantages: The YR06248 and YR06249 models offer superior temperature accuracy and uniformity, high throughput, and advanced optical systems. Their ability to perform multiplexing and detect single gene copies makes them indispensable for complex analyses.
Disadvantages: Despite their advanced features, the systems may require routine calibration and maintenance. Novice users may need initial training to make full use of the systems’ capabilities.
Product Use in the Field
These real-time PCR systems are extensively utilized in research laboratories for gene expression studies, SNP genotyping, and pathogen detection. In clinical diagnostics, they play a crucial role in detecting microbial agents and monitoring disease progression. Their dynamic range and sensitivity are especially beneficial in fields requiring precise quantitation and analysis of genetic material.
In academic settings, the flexibility in sample and dye compatibility makes them an invaluable tool for experimental research and training labs. Moreover, their robust design ensures reliability even under high throughput and stringent laboratory conditions.
Recommendations
To obtain the best results, it is recommended to perform regular maintenance checks and calibrate the equipment periodically. Users should ensure that they follow appropriate laboratory protocols and equipment guidelines to maximize the utility of the systems.
Additionally, investing in proper training for lab personnel can optimize system use, thereby improving work efficiency and data quality. Implementing the use of high-standard consumables can further enhance the performance and longevity of these sophisticated PCR machines.
If you’re looking for a fusion of innovation and quality, you’ve come to the right place. At Kalstein, we offer you the luxury of exploring our exclusive catalog of laboratory equipment. We manufacture each piece with a level of excellence. Our intuitive and agile online purchasing channels are designed for your convenience, ensuring the most friendly prices. Don’t hesitate anymore, we give life to science, it’s time to become part of our community. https://kalstein.net/en/product/real-time-quantitative-pcr-system-yr06248-yr06249/.
