Product Description
The real-time quantitative PCR systems, models YR06248 and YR06249, are at the forefront of modern scientific research. These PCR systems offer unparalleled accuracy and efficiency, designed to meet the dynamic needs of contemporary laboratories. With a sample capacity that accommodates 8 x 0.1mL PCR strips x 12, 96 x 0.1mL individual tubes, and 0.1mL PCR plates, they scale perfectly for various experimental needs.
The systems operate within a sample volume range of 10-50 μL, supported by a Peltier heating and cooling method, ensuring swift and reliable thermal cycling. The maximum ramping rate of 6.0°C per second coupled with a temperature range of 4-100°C guarantees rapid processing times and high-throughput performance.
Both models boast a temperature accuracy of ±0.2°C, emphasizing their reliability in producing consistent results. The use of SiPMT detectors and time-resolved real-time scanning enhances the precision of data collection, while the range of supported dyes extends their versatility across various assays.
| Model | YR06248 | YR06249 |
| Sample capacity | 8*0.1mL PCR strip*12 96*0.1mL individual tube 0.1mL PCR Plate |
|
| Sample volume | 10-50 μL | |
| Heating and Cooling method | Peltier | |
| Maximum ramping rate | 6.0° C/s | |
| Temperature range | 4-100 °C | |
| Temperature accuracy | ± 0.2°C | |
| Temperature uniformity | ±0.2°C@60°C ±0.3°C@95°C | |
| Optical Detecting System | ||
|---|---|---|
| Excitation light | 4pcs high-efficiency single color LED | 5pcs high-efficiency single color LED |
| Detector | SiPMT | |
| Detection method | Time-resolved real-time scanning | |
| Excitation/Emission wavelengths | 455-650nm | 510-750nm |
| Detection Channels | 4 (optional 5 channels） | |
| Supported dyes | FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ，（Optional- Cy5.5 for 5-channel model） | |
| Multiplexing | Up to 4 targets (optional 5 targets for 5-channel model) | |
| Sensitivity | 1 copy gene | |
| Resolution | 1.33-fold copies difference in single-plex reaction | |
| Dynamic range | 10 orders of magnitude | |
Market Price
The revolutionary YR06248 and YR06249 real-time PCR systems are strategically priced to be competitive within the market. The typical range for equipment of this caliber is approximately $23,500 to $23,800, placing them well within the reach of research institutions and clinical laboratories aiming to enhance their molecular analysis capabilities. The innovative features and precision these models offer justify the investment, making them an excellent addition to any well-equipped laboratory.
Frequently Asked Questions
What is the main difference between the YR06248 and YR06249 models?
The primary difference lies in the optical system: YR06248 is equipped with 4 high-efficiency single-color LEDs, while YR06249 features 5, providing a broader excitation light range, thus expanding detection capabilities.
How does the temperature accuracy benefit my experiments?
With an accuracy of ±0.2°C, these systems ensure precise and reproducible results, critical for assays requiring exact temperature conditions, notably in sensitive molecular analyses.
What are the supported dyes, and how do they enhance flexibility?
Both models support a wide range of dyes including FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ, with the option of Cy5.5 in 5-channel models, making them suitable for diverse applications and experiments.
Advantages and Disadvantages
Advantages:
These PCR systems offer high sensitivity, detecting down to a single copy gene, and exceptional resolution with a 1.33-fold copy difference in single-plex reactions. The dynamic range extends over 10 orders of magnitude, making them capable of handling complex analyses. With LED excitation and SiPMT detection, they provide excellent data precision and reliability.
Disadvantages:
Despite their high performance, the systems may require careful calibration and setup to maximize their capabilities. The initial investment might be a barrier for smaller labs, although the long-term benefits often outweigh the upfront costs.
Product Use in the Field
In practical applications, the YR06248 and YR06249 models are pivotal in genetic research, clinical diagnostics, and biotechnological developments. They provide reliable and precise results rapidly, supporting genomic studies and pathogen detection with ease. Their robustness and adaptability lend themselves well to high-throughput environments, driving innovation in genomic research and personalized medicine.
Recommendations
To ensure optimal performance and longevity of your PCR system, regular maintenance and calibration are recommended. Use the appropriate consumables and follow proper protocols for sample preparation to maximize data quality. Additionally, leveraging Kalstein Plus can streamline equipment procurement, ensuring you receive support and updates directly from the manufacturer, enhancing your laboratory’s efficiency.
If you’re seeking a fusion of innovation and quality, you’ve come to the right place. At Kalstein, we offer you the luxury of exploring our exclusive catalog of laboratory equipment. We manufacture each equipment with a high level of excellence. Our intuitive and agile online shopping channels are designed for your convenience, ensuring the friendliest prices. Don’t hesitate any longer, we bring science to life, it’s time to become part of our community. https://kalstein.net/en/product/real-time-quantitative-pcr-system-yr06248-yr06249/.
