Product Description
The YR06248 and YR06249 models represent the cutting-edge of real-time quantitative PCR technology, designed to meet the demanding needs of modern laboratories. Both models are equipped with a robust sample capacity, capable of handling 8 PCR strips of 0.1 mL each across 12 slots, or utilizing 96 individual 0.1 mL PCR tubes and a 0.1 mL PCR plate. This flexibility ensures compatibility with a wide range of sample preparations, making it an ideal choice for diverse applications in genetic research, clinical diagnostics, and more.
The precision of the YR06248 and YR06249 systems is underscored by their Peltier heating and cooling mechanism, which facilitates a maximum ramping rate of 6.0°C/s. This, coupled with a temperature range of 4-100°C and an accuracy of ± 0.2°C, ensures impeccable results in every cycle. With temperature uniformity maintained at ±0.2°C at 60°C and ±0.3°C at 95°C, these models guarantee consistent and reliable outcomes in real-time PCR assays.
| Model | YR06248 | YR06249 |
| Sample capacity | 8*0.1mL PCR strip*12 96*0.1mL individual tube 0.1mL PCR Plate |
|
| Sample volume | 10-50 μL | |
| Heating and Cooling method | Peltier | |
| Maximum ramping rate | 6.0° C/s | |
| Temperature range | 4-100 °C | |
| Temperature accuracy | ± 0.2°C | |
| Temperature uniformity | ±0.2°C@60°C ±0.3°C@95°C | |
| Optical Detecting System | ||
| Excitation light | 4pcs high-efficiency single color LED | 5pcs high-efficiency single color LED |
| Detector | SiPMT | |
| Detection method | Time-resolved real-time scanning | |
| Excitation/Emission wavelengths | 455-650nm | 510-750nm |
| Detection Channels | 4 (optional 5 channels) | |
| Supported dyes | FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ, (Optional- Cy5.5 for 5-channel model) | |
| Multiplexing | Up to 4 targets (optional 5 targets for 5-channel model) | |
| Sensitivity | 1 copy gene | |
| Resolution | 1.33-fold copies difference in single-plex reaction | |
| Dynamic range | 10 orders of magnitude | |
Market Price
The state-of-the-art real-time PCR systems such as the YR06248 and YR06249 are priced competitively within the market, offering tremendous value for their advanced features and capabilities. A typical price range in the market for these models begins at approximately $22,500 USD and can extend up to $23,800 USD, depending on the configuration and any additional features selected. This pricing reflects the exceptional quality and high performance of these instruments, catering to laboratories that prioritize accuracy and efficiency in their workflows.
Frequently Asked Questions
What sample volume is supported by the YR06248 and YR06249 models?
These PCR systems accommodate a sample volume ranging from 10 to 50 μL, ensuring flexibility across various experimental requirements.
Can these models handle multiplex reactions?
Yes, both models support multiplexing for up to 4 targets, and an optional configuration allows for 5-channel multiplexing.
What type of detection method is utilized in these PCR systems?
The YR06248 and YR06249 models employ a time-resolved real-time scanning detection method with high sensitivity and precision.
Advantages and Disadvantages
Advantages: The YR06248 and YR06249 offer excellent temperature control and uniformity, critical for precise PCR results. Their multiplex capabilities and broad dynamic range simplify complex analyses, while their LED-based optical system ensures reliable and efficient detection.
Disadvantages: As high-performance instruments, these models might represent a higher initial investment, making them suitable primarily for laboratories focused on cutting-edge research and routine high-throughput applications.
Field Use of the Product
In clinical and research laboratories, the YR06248 and YR06249 are essential for real-time PCR assays, widely used in pathogen detection, gene expression analysis, and genotyping. Their robustness and precision ensure these instruments are well-suited for critical diagnostic settings, providing reliable and reproducible data.
By supporting a variety of dyes and offering time-resolved scanning, these devices facilitate in-depth analyses, meeting the demands of cutting-edge research and clinical diagnostics alike.
Recommendations
To maximize the utility and lifespan of your YR06248 or YR06249 system, ensure regular maintenance and calibration according to the manufacturer’s guidelines. Use high-quality reagents and consumables to maintain the integrity of the instrument and the accuracy of results. It’s also recommended to engage in regular training and updates to stay abreast of the latest developments and techniques in PCR technology.
If you are looking for a fusion of innovation and quality, you’ve come to the right place. At Kalstein, we offer you the luxury of exploring our exclusive catalog of laboratory equipment. We manufacture each device with a level of excellence. Our intuitive and agile online purchasing channels are designed for your convenience, ensuring the friendliest prices. Don’t hesitate any longer, we bring science to life, it’s time to be part of our community. https://kalstein.net/en/product/real-time-quantitative-pcr-system-yr06248-yr06249/.
