The process of choosing a thermal cycler requires a thorough analysis of the technical specifications of each device. Manufacturers consider factors such as dimensions, weight, consumable compatibility, reaction volume, among other parameters.
As a manufacturer, Kalstein offers the YR01867 model with dimensions of 205mm(L)*156mm(W)*153mm(H) and a weight of 3.2kg. The device is compatible with individual 0.2mL clear tubes and strip tubes of 8. It has a yield from 1 to 16 and a reaction volume from 0 to 100ul. https://kalstein.net/en/product/thermal-cyclers-yr01867/
On the other hand, GeneSig presents the model genesig q16 Real-Time PCR Instrument, which is small and light, weighing only 2 kg and accommodating 16 0.2ml tubes. Bioer Technology, with its LineGene Mini model, offers dimensions of 280mm(L)*220mm(W)*240mm(H))) and a weight of 6.5kg. Finally, Coyote stands out for its Mini8 plus model which is notably compact and lightweight, with a weight of 2.1Kg.
Advantages and disadvantages of Kalstein in comparison
The advantages of buying a Kalstein thermal cycler stand out for its ability to achieve high performance with up to 16 consumables, while the Coyote model only handles 8. Although Kalstein and Bioer handle a similar range of reaction volume, the advantage of Kalstein is that it can handle volumes as low as 0ul, which can result in potential savings of supplies and therefore, price optimization in some lab procedures.
The disadvantage lies in the weight of the Kalstein device against GeneSig and Coyote. In terms of performance and reaction volume handling capacity, Bioer Technology matches Kalstein.
Thermal performance
Thermal performance is crucial for the success of various lab procedures. Kalstein leads in this area with an average heating rate of 3.3℃/s and a cooling rate of 3.0℃/s, ahead of Coyote with >1.5°C/s. However, its competition equals or surpasses the maximum heating and cooling rates of 5.0°C/s and 4.0°C/s, respectively.
Optical options and warranty
In terms of optical options, Kalstein stands out with 4 fluorescence channels and a wide range of dye compatibility. It offers a 2-year warranty, surpassing the rest that offers 1 year.
In summary, the sale of thermal cyclers should contemplate both technical characteristics and the advantages and disadvantages of each manufacturer. In this sense, it is clear that Kalstein is a competitive option due to its performance, reaction volume, optical options, and warranty.