Product Description
The YR01869 Thermal Cycler by Kalstein is a state-of-the-art instrument designed to meet the demands of modern molecular biology laboratories. This advanced thermal cycler is equipped with remarkable features that cater to high-throughput scenarios, offering capabilities often sought in cutting-edge research settings. With its inituitive interface and robust performance, the YR01869 stands as a vital asset for researchers worldwide.
Key specifications include a throughput of up to 96 samples and a comprehensive array of fluorescence channels totaling six, providing versatility in dye compatibility. These channels are meticulously designed to cater to a range of fluorophores including FAM, SYBR Green I, ROX, and Cy5, among others. The fluorescence scanning time is efficiently executed in just seven seconds, making it ideal for fast-paced labs.
The optical system is enhanced with a high-brightness, long-life LED light source that requires no maintenance and conducts excitation from the top. Accompanied by photodiodes (PDs) for top scanning, the excitation and detection ranges are carefully calibrated for precision. The thermal management of the YR01869 is outstanding, boasting a Peltier heating method with rapid heating and cooling rates and temperature accuracy of ±0.1 ℃. The programmable gradient range introduces flexibility, catering to various PCR methodologies such as Touch Down and Long PCR, ensuring versatility for innumerable experimental requirements.
| Model | YR01869 |
| Throughput | Up to 96 samples |
| Fluorescence Channels | 6 |
| Fluorescence Scanning Time | 7s |
| Dye Compatibility | Channel 1: FAM, SYBR Green I, SYTO 9, Eva Green, LC Green |
| Channel 2: HEX, VIC, TET, JOE | |
| Channel 3: ROX, Texas Red | |
| Channel 4: Cy5 | |
| Channel 5: Alexa Fluor 680 | |
| Channel 6: FRET | |
| Optical System | |
| Light Source | High-brightness, long-life and maintenance-free LED light source, excitation from the top |
| Detector | Photodiodes (PDs), top scanning |
| Excitation Range | CH1: 465nm CH2:527nm CH3:580nm CH4:632nm CH5:680nm CH6:465nm |
| Detection Range | CH1: 510nm CH2:563nm CH3:616nm CH4:664nm CH5:730nm CH6:616nm |
| Fluorescence Dynamic Range | Adjustable |
| Sample Dynamic Range | From 101 to 1010 copies |
| Thermal Black | |
| Heating Method | Peltier |
| Heating Rate | >6.1 ℃/s |
| Cooling Rate | >5.0 ℃/s |
| Temperature Uniformity | ±0.1 ℃ |
| Temperature Accuracy | ≤0.1 ℃ |
| Gradient Range | 1℃-40 ℃ |
| Gradient Block | 12 row |
| Special Temperature Protocol | Thermal gradients PCR, long PCR, Touch Down PCR |
| Sample Testing Linearity and Repeatability | Linear correlation: /r/ ≥ 0.999 |
| Repeatability: cycle threshold (Ct) value CV≤0.5% | |
| Software Functions | |
| Control Modes | Model 1: 10.4 inch touch screen |
| Mode 2: PC direct control | |
| Power Failure Protection | Automatically start running experiments after power supply, no need to wait for PC software |
| Data Storage and Transmission | Upload and download through USB disk. 1000 results can be stored in the machine |
| Reporting Function | Templates reserved; customized experiment report |
| Key Applications | Relative quantification, absolute quantification, melting curve analysis, SNP analysis |
| Others | |
| Operating System for PC | Win7/Win10/Win11 |
| Power Supply and Power Consumption | AC 100-240V, 50-60Hz, 900VA |
| Weight | 30 Kg (net) |
| Instrument Dimension | 555*475*484mm (W*L*H) |
| Suitable Consumables | 0.2mL 96-well plates, 8-tube strips, single tubes (clear, frosted and white). |
Market Price
In today’s competitive market, thermal cyclers like the YR01869 are typically priced between USD 28,500 and USD 29,400. This pricing reflects the remarkable capabilities and high performance that such an instrument offers, making it an invaluable investment for laboratories intent on achieving precision and reliability in their molecular biology assays.
Frequently Asked Questions
What is the throughput capacity of the YR01869 Thermal Cycler?
The YR01869 can process up to 96 samples simultaneously, making it suitable for high-volume laboratories.
Is the thermal cycler compatible with multiple types of dyes?
Yes, this model supports a wide range of dyes through its six fluorescence channels, including FAM, SYBR Green I, ROX, Cy5, and more.
Can the instrument function without a PC?
Indeed, the YR01869 can operate in standalone mode via its 10.4-inch touch screen, but it also supports direct PC control if needed.
Advantages and Disadvantages
Advantages: The YR01869 Thermal Cycler is highly versatile due to its extensive dye compatibility, rapid scanning time, and high throughput capacity. The advanced thermal management and precise temperature control systems ensure the highest accuracy and reliability for PCR experiments. Additionally, the dual control modes provide flexibility for users preferring direct PC interface or standalone operation.
Disadvantages: Although the device offers a host of advanced features, its sophisticated technology might require a learning curve for new users unfamiliar with high-tech lab equipment. Additionally, initial costs are higher compared to basic models, reflecting its enhanced capabilities.
Usage in the Field
The YR01869 is ideally suited for various applications in the field of molecular biology and genetics. In practical settings, it is extensively used for quantitative PCR, high-resolution melting curve analysis, and SNP genotyping. Its rapid processing times and high accuracy make it invaluable for both research and diagnostic laboratories, where precise quantification and analysis are critical.
For research involving genetic material, the facilitated dye compatibility and rapid fluorescence scanning enable detailed analysis and high-throughput data acquisition, essential for large-scale projects and time-sensitive diagnostics.
Recommendations
To ensure optimal performance of the YR01869 Thermal Cycler, regular calibration and maintenance checks are advised. Users should familiarize themselves with the multi-functional control interface to take full advantage of its capabilities. Additionally, employing high-quality consumables compatible with its dye and throughput specifications is crucial for achieving reliable results.
Leverage the expertise of Kalstein’s support team for any technical queries to further enhance your laboratory’s productivity and assay precision. Interested buyers are encouraged to request a quote or generate an instant quote using the Kalstein Plus platform to explore customized purchasing options.
If you’re seeking a fusion of innovation and quality, you’ve reached the right place. At Kalstein we offer you the luxury of exploring our exclusive catalog of laboratory equipment. We manufacture each device with a level of excellence. Our intuitive and agile online purchasing channels are designed for your convenience, ensuring the most user-friendly prices. Don’t hesitate any longer, we bring science to life, it’s time to be part of our community. https://kalstein.net/en/product/thermal-cyclers-yr01869/.
