Product Description
The YR01869 thermal cycler is a state-of-the-art laboratory equipment designed to enhance the efficiency and accuracy of PCR experiments. This high-throughput instrument is equipped with six fluorescence channels, allowing it to simultaneously process multiple samples in parallel, thereby increasing the laboratory’s output. The fluorometric scanning time is an impressive 7 seconds, ensuring rapid data acquisition without compromising precision.
One of the remarkable features of the YR01869 is its wide dye compatibility across its channels, supporting a range of dyes from FAM and SYBR Green I to Alexa Fluor 680 and FRET. This diverse dye compatibility ensures that the instrument can be used for various molecular biology applications, from basic DNA quantification to complex melting curve analyses.
Additionally, the optical system of the YR01869, which utilizes a high-brightness, long-life LED light source, requires minimal maintenance and offers top excitation. Coupled with photodiodes for top scanning, this system provides broad excitation and detection ranges that facilitate precise measurements across a range of wavelengths.
| Model | YR01869 |
| Throughput | 1-96 |
| Fluorescence Channels | 6 |
| Fluorescence Scanning Time | 7s |
| Dye Compatibility | Channel 1: FAM, SYBR Green I , SYTO 9, Eva Green, LC Green |
| Channel 2: HEX, VIC, TET, JOE | |
| Channel 3: ROX, Texas Red | |
| Channel 4: Cy5 | |
| Channel 5: Alexa Fluor 680 | |
| Channel 6: FRET | |
| Optical System | |
| Light Source | High-brightness, long life and maintenance-free LED light source, excitation from the top |
| Detector | Photodiodes (PDs), top scanning |
| Excitation Range | CH1: 465nm CH2:527nm CH3:580nm CH4:632nm CH5:680nm CH6:465nm |
| Detection Range | CH1: 510nm CH2:563nm CH3:616nm CH4:664nm CH5:730nm CH6:616nm |
| Fluorescence Dynamic Range | Adjustable |
| Sample Dynamic Range | From 101 to 1010 copies |
| Thermal Black | |
| Heating Method | Peltier |
| Heating Rate | >6.1 ℃/s |
| Cooling Rate | >5.0 ℃/s |
| Temperature Uniformity | ±0.1 ℃ |
| Temperature Accuracy | ≤0.1 ℃ |
| Gradient Range | 1℃-40 ℃ |
| Gradient Block | 12 row |
| Special Temperature Protocol | Thermal gradients PCR, long PCR, Touch Down PCR |
| Sample Testing Linearity and Repeatability | Linear correlation: /r/ ≥ 0.999 |
| Repeatability: cycle threshold (Ct) value CV≤0 0.5% | |
| Software Functions | |
| Control Modes | Model 1: 10.4 inch touch screen |
| Mode 2: PC direct control | |
| Power Failure Protection | Automatically start running experiments after power supply, no need to wait PC software |
| Data Storage and Transmission | Upload and download through USB disk. 1000 results can be stored in machine |
| Reporting Function | Templates reserved; customized experiment report |
| Key Applications | Relative quantification, absolute quantification, melting curve analysis, SNP analysis |
| Others | |
| Operating System for PC | Win7/Win10/Win11 |
| Power Supply and Power Consumption | AC 100-240V,50-60HZ, 900VA |
| Weight | 30 Kg (net) |
| Instrument Dimension | 555*475*484mm (W*L*H) |
| Suitable Consumables | 0.2mL 96-well plates, 8-tube strips, single tubes (clear, frosted and white). |
Market Price
The YR01869 thermal cycler occupies a premium position in the market, reflecting its advanced technology and multifunctionality. This laboratory essential typically ranges between $29,000 and $30,500, a competitive price segment when weighed against its innovative features and reliable performance. Such pricing provides labs with a high return on investment due to enhanced efficiency and productivity in experiments. We invite you to request a personalized quote directly through the Kalstein website for precise pricing tailored to your region and specific needs.
Frequently Asked Questions
What is the throughput capacity of the YR01869 thermal cycler?
The YR01869 can process up to 96 samples concurrently, making it ideal for high-throughput laboratories.
What are the control options available for this device?
The thermal cycler offers two primary control modes: a 10.4-inch touch screen for direct operation and PC direct control for remote management.
Can I retrieve experiment data without a connected PC?
Yes, the device allows data upload and download through a USB disk and can store up to 1000 results, making PC connection optional for data retrieval.
Advantages and Disadvantages
Advantages: The YR01869 boasts an array of beneficial features, such as rapid heating and cooling rates of over 6.1 ℃/s and 5.0 ℃/s respectively, which significantly enhances workflow efficiency. It uses a maintenance-free LED light source and comes with the flexibility of accommodating a broad spectrum of dyes, expanding its applicability across numerous PCR applications.
Disadvantages: While its features are advanced, users must consider its weight, which at 30 kg, may require designated laboratory space. Additionally, the initial investment may seem substantial for smaller labs, although this cost is offset by its high throughput capabilities and reliable performance.
Field Use of the Product
In practical scenarios, the YR01869 thermal cycler is invaluable for research settings that require precise thermal controls, such as genetic testing, pathogen detection, or any DNA-based analyses. Its ability to maintain tight temperature uniformity (±0.1 ℃) and accuracy (≤0.1 ℃) ensures reproducibility and reliability of results, critical for sensitive applications like SNP analysis and quantitative PCR assays.
The device supports specialized PCR techniques, including thermal gradient PCR, long PCR, and touchdown PCR, enabling researchers to conduct a broad range of experiments under varying thermal conditions. Its robust design and intuitive interface enhance user experience, making it a trusted asset in any laboratory.
Recommendations
For optimal results, it is recommended that users regularly calibrate the thermal cycler to maintain its precision and accuracy over time. Utilizing high-quality consumables like 0.2mL 96-well plates is advisable for best outcomes. Regular cleaning and maintenance checks will preserve the system’s longevity and performance.
Considering its features and applications, the YR01869 thermal cycler is best suited for labs engaged in complex genetic analyses and high-throughput screenings. New users are encouraged to train familiarization sessions to maximize the equipment’s potential and ensure seamless integration into existing workflows.
If you’re searching for a blend of innovation and quality, you’ve come to the right place. At Kalstein, we offer you the luxury of exploring our exclusive catalog of laboratory equipment. We manufacture each piece with a level of excellence. Our online purchasing channels, intuitive and agile, are designed for your convenience, ensuring the most friendly prices. Don’t hesitate any longer; we bring science to life, and it’s time to become part of our community. https://kalstein.net/en/product/thermal-cyclers-yr01869/.
