In the realm of molecular biology, the Real-Time Quantitative PCR System YR06248 and YR06249 stand out as exemplary models, bringing precision and efficiency to a new level. These systems are intricate yet user-friendly, designed to ensure that scientific analysis is both reliable and reproducible. With a combination of advanced heating and detection technologies, these PCR systems cater to laboratories aiming for cutting-edge results.
Description of the Product
The YR06248 and YR06249 models are designed with a robust sample capacity, accommodating 96 x 0.1mL individual tubes or plates, to suit various research needs. This flexibility in sample accommodation makes them an adaptable choice for both high-throughput and smaller-scale projects. Featuring an effective sample volume range of 10-50 μL, these systems cater to various experimental requirements efficiently.
The systems utilize a Peltier-based heating and cooling method, distinguished by a maximum ramping rate of 6.0°C/s. This high ramping rate enables quick thermal cycling, which is vital for minimizing the time required for PCR reactions. With a broad temperature range of 4-100 °C, the systems ensure precise control over the reaction conditions, essential for achieving accurate results.
Temperature accuracy and uniformity are critical in PCR processes, and these systems do not disappoint. With an accuracy of ±0.2°C and uniformity of ±0.2°C@60°C to ±0.3°C@95°C, the YR06248 and YR06249 ensure that each sample receives the same thermal conditions. This precision is complemented by a sophisticated Optical Detecting System, which features single-color high-efficiency LEDs ranging from 4 to 5 pieces, SiPMT detectors, and time-resolved real-time scanning, ensuring accurate data capture.
The following table provides a detailed overview of their technical specifications:
| Model | YR06248 | YR06249 | |
|---|---|---|---|
| Sample capacity | 8*0.1mL PCR strip*12, 96*0.1mL individual tube, 0.1mL PCR Plate | ||
| Sample volume | 10-50 μL | ||
| Heating and Cooling method | Peltier | ||
| Maximum ramping rate | 6.0°C/s | ||
| Temperature range | 4-100 °C | ||
| Temperature accuracy | ± 0.2°C | ||
| Temperature uniformity | ±0.2°C@60°C ±0.3°C@95°C | ||
| Optical Detecting System | Excitation light | 4pcs high-efficiency single color LED | 5pcs high-efficiency single color LED |
| Detector | SiPMT | ||
| Detection method | Time-resolved real-time scanning | ||
| Excitation/Emission wavelengths | 455-650nm | 510-750nm | |
| Detection Channels | 4 (optional 5 channels） | ||
| Supported dyes | FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ，（Optional- Cy5.5 for 5-channel model） | ||
| Multiplexing | Up to 4 targets (optional 5 targets for 5-channel model) | ||
| Sensitivity | 1 copy gene | ||
| Resolution | 1.33-fold copies difference in single-plex reaction | ||
| Dynamic range | 10 orders of magnitude | ||
Market Price
The Real-Time Quantitative PCR Systems YR06248 and YR06249 are positioned in the premium segment of the market, with prices typically ranging from $22,500 to $23,800. These figures reflect the advanced technology and precise engineering embodied in each unit, making them a worthwhile investment for laboratories prioritizing quality and accuracy.
Frequently Asked Questions
What is the sample capacity of these PCR systems?
Both models can handle 8*0.1mL PCR strips*12, 96*0.1mL individual tubes, or a 0.1mL PCR plate.
How does the optical detecting system work?
The optical detecting system uses high-efficiency single color LEDs and SiPMT detectors with time-resolved real-time scanning for precise fluorescence detection.
What dyes are supported by these systems?
The systems support a range of dyes including FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ, and optionally Cy5.5 for the 5-channel model.
Advantages and Disadvantages
Advantages: The YR06248 and YR06249 models boast a highly sensitive detection system capable of detecting as low as 1 copy gene, a wide dynamic range of 10 orders of magnitude, and a remarkable resolution for single-plex reactions. Additionally, their precision in temperature control enhances all types of PCR applications, ensuring reproducibility and consistency.
Disadvantages: As with most high-end laboratory equipment, the cost may be a consideration for smaller research facilities. Moreover, the complexity of the equipment might require more training for lab personnel to utilize all features effectively.
Field Use of the Product
In practical laboratory settings, the YR06248 and YR06249 PCR systems are utilized for a myriad of applications, ranging from pathogen detection to genotyping experiments. Their real-time scanning capability allows for the continuous monitoring of reaction progress, providing researchers with insightful data for immediate analysis and decision-making. Furthermore, the systems’ compatibility with various dye formats expands their applicability across different types of molecular assays.
Researchers worldwide appreciate these systems’ efficiency and consistency, which translate into increased throughput and reduced time-to-results. This allows labs to streamline workflows, thereby enhancing research productivity and enabling breakthroughs.
Recommendations
To maximize the performance of the YR06248 and YR06249 PCR systems, it is recommended to routinely calibrate the instrument and perform maintenance checks as per the manufacturer’s guidelines. Utilizing compatible reagents and consumables is also crucial for maintaining the integrity and accuracy of test results. Training laboratory staff on the functionality and operation of these systems can further enhance the quality and consistency of experimental outcomes.
Scientists seeking a blend of innovation and precision in their PCR analysis are encouraged to explore the functionalities of these PCR systems. Leveraging these tools can significantly elevate research initiatives, prompting discoveries and advancements in genetic studies. For more in-depth specifications or to get a personalized quote, consider generating an automatic quotation through Kalstein Plus today. If you are looking for a fusion of innovation and quality, you have come to the right place. At Kalstein we offer you the luxury of exploring our exclusive catalog of laboratory equipment. We manufacture each equipment with a level of excellence. Our intuitive and agile online purchasing channels are designed for your convenience, ensuring the friendliest prices. Do not hesitate any longer, we give life to science, it is time to become part of our community. https://kalstein.net/en/product/real-time-quantitative-pcr-system-yr06248-yr06249/.
