Product Description
The YR01869 Thermal Cycler stands as a testament to cutting-edge biotechnology. Designed to excel in laboratory settings, this thermal cycler offers high throughput capabilities suitable for rigorous applications. It boasts masterful integration of technological advancements, evidenced by its six fluorescence channels and an impressive fluorescence scanning time of just 7 seconds. The versatility in dye compatibility, with options including FAM, SYBR Green I, and others, ensures diverse utility across different experimental needs.
The machine’s optical system is headlined by a high-brightness, long-life, maintenance-free LED light source, providing exceptional excitation from the top. Meanwhile, its detector consists of photodiodes (PDs) designed for top scanning, offering comprehensive excitation and detection ranges extending across multiple channels. Furthermore, the Peltier heating method ensures a rapid >6.1 ℃/s heating rate combined with a >5.0 ℃/s cooling rate, promising consistent performance through its precision temperature control capabilities.
| Model | YR01869 |
| Throughput | 1-96 |
| Fluorescence Channels | 6 |
| Fluorescence Scanning Time | 7s |
| Dye Compatibility | Channel 1: FAM, SYBR Green I , SYTO 9, Eva Green, LC Green |
| Channel 2: HEX, VIC, TET, JOE | |
| Channel 3: ROX, Texas Red | |
| Channel 4: Cy5 | |
| Channel 5: Alexa Fluor 680 | |
| Channel 6: FRET | |
| Optical System | |
| Light Source | High-brightness, long life and maintenance-free LED light source,excitation from the top |
| Detector | Photodiodes (PDs), top scanning |
| Excitation Range | CH1: 465nm CH2:527nm CH3:580nm CH4:632nm CH5:680nm CH6:465nm |
| Detection Range | CH1: 510nm CH2:563nm CH3:616nm CH4:664nm CH5:730nm CH6:616nm |
| Fluorescence Dynamic Range | Adjustable |
| Sample Dynamic Range | From 101 to 1010copies |
| Thermal Black | |
| Heating Method | Peltier |
| Heating Rate | >6.1 ℃/s |
| Cooling Rate | >5.0 ℃/s |
| Temperature Uniformity | ±0.1 ℃ |
| Temperature Accuracy | ≤0.1 ℃ |
| Gradient Range | 1℃-40 ℃ |
| Gradient Block | 12 row |
| Special Temperature Protocol | Thermal gradients PCR, long PCR, Touch Down PCR |
| Sample Testing Linearity and Repeatability | Linear correlation: /r/ ≥ 0.999 |
| Repeatability: cycle threshold (Ct) value CV≤0 0.5% | |
| Software Functions | |
| Control Modes | Model 1: 10.4 inch touch screen |
| Mode 2: PC direct control | |
| Power Failure Protection | Automatically start running experiments after power supply, no need to wait for PC software |
| Data Storage and Transmission | Upload and download through USB disk. 1000 results can be stored in machine |
| Reporting Function | Templates reserved; customized experiment report |
| Key Applications | Relative quantification, absolute quantification, melting curve analysis, SNP analysis |
| Others | |
| Operating System for PC | Win7/Win10/Win11 |
| Power Supply and Power Consumption | AC 100-240V, 50-60HZ, 900VA |
| Weight | 30 Kg (net) |
| Instrument Dimension | 555*475*484mm (W*L*H) |
| Suitable Consumables | 0.2mL 96-well plates, 8-tube strips, single tubes(clear, frosted and white). |
Market Price
In the current market, premium thermal cyclers such as the YR01869 typically range between $27,380 and $29,980. Pricing considerations often involve the innovative features, the number of fluorescence channels, and precision engineering that offer advantageous results in scientific exploration. Thus, while the upfront cost might seem substantial, the sustained performance and reliability delivered by the YR01869 make it a significant investment for any research facility.
Price variations might occur depending on regional distribution partnerships and additional bundled offers, particularly where consumable parts or extended service plans are included. Take advantage of Kalstein Plus to access tailored pricing and expedited quotations, ensuring that your procurement fits seamlessly within your budgetary constraints.
Frequently Asked Questions
What is the throughput of the YR01869 Thermal Cycler?
The YR01869 offers a throughput of 1-96, accommodating various sample volumes and types, thus providing flexibility in experimental setups.
Can it store experimental data?
Yes, the YR01869 can store up to 1000 results internally, with data transfer capabilities through USB disk for further analysis or archiving.
What dye types can this cycler detect?
It offers extensive dye compatibility across its six channels, including FAM, SYBR Green I, Cy5, and FRET, among others, thereby supporting a wide range of fluorescence-based assays.
Advantages and Disadvantages
The YR01869 thermal cycler shines with its multi-channel fluorescence detection, rapid heating and cooling rates, and superior temperature control precision. These features ensure high reliability and discrimination in assays, especially in fields requiring quantitative research and SNP analysis. Additionally, the control flexibility provided by both touchscreen and PC software enhances its user-friendly design.
However, some laboratories with modest throughput needs might find its advanced specifications surplus to their daily requirements, and therefore, might opt for simpler, more economical models. Maintenance-wise, the longevity and maintenance-free design of core components such as the LED light source minimize downtime, though technical intervention may still be required for calibration over time.
Field Use
In practical terms, the YR01869 is a workhorse in molecular biology and genetic research. It facilitates both relative and absolute quantification tasks, as well as precise melting curve analysis for genotyping and quality assessment. Its use in real-world laboratory settings demonstrates its utility during intensive workflows, spanning basic research to high-uniformity diagnostic applications.
Its programmable thermal gradients and specialized protocols like Touch Down PCR empower researchers to optimize results tailored to specific requirements, ensuring it meets the stringent demands of contemporary molecular experimentation.
Recommendations
For optimized performance, it is recommended to operate the YR01869 in compliance with factory settings regarding power supply and environmental conditions. Regular software updates, easily facilitated via the USB storage system, help maintain its cutting-edge functionality. Furthermore, ensuring correct consumable compatibility, such as using 0.2mL 96-well plates, is essential to achieving accurate and reliable results.
Institutions intending extensive utility from the YR01869 should consider integrating it within a larger ecosystem of compatible lab instruments, thereby enhancing throughput while minimizing setup time per assay. Engage with the Kalstein Plus platform for personalized advice and expert support to make the most of your investment.
If you are looking for a fusion of innovation and quality, you have come to the right place. At Kalstein we offer you the luxury of exploring our exclusive catalog of laboratory equipment. We manufacture each equipment with a level of excellence. Our intuitive and agile online shopping channels are designed for your convenience, ensuring the most friendly prices. Don’t hesitate any longer, we bring science to life, it’s time for you to be part of our community. https://kalstein.net/en/product/thermal-cyclers-yr01869/.
